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  • Articles: DFG German National Licenses  (3)
  • Genetic analysis  (2)
  • Aspergillus nidulans  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Adenine and pyrimidine genes of Aspergillus niger and evidence for a seventh linkage group (1989)
    Springer
    Current genetics 16 (1989), S. 307-310 
    Details
    ISSN: 1432-0983
    Keywords: Aspergillus niger ; Adenine mutants ; Pyrimidine mutants ; Linkage groups ; Genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of Aspergillus niger requiring adenine and one mutant requiring cytosine were isolated after low-dose mutagenesis and enrichment. In addition we had mutants of two genes involved in the pyrimidine biosynthesis isolated as 5-fluoro-orotic acid-resistant mutants. The fifteen adenine-less mutants could be placed in seven complementation groups. From each group a representative mutant was analyzed in order to determine the linkage group by analysis of the mutants in a heterozygous diploid carrying markers in six linkage groups. AdeF could not be assigned to any one of these linkage groups and proved to be linked to nicB, oliC and cnxC, none of which could be placed in a linkage group. Thus, conclusive evidence was obtained for a seventh linkage group. As pyrA was used as selection marker for transformation, we constructed a pyrA strain with a linked marker which can be used in the genetic analysis of transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422118
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Induction and isolation of mutants in fungi at low mutagen doses (1987)
    Springer
    Current genetics 12 (1987), S. 471-474 
    Details
    ISSN: 1432-0983
    Keywords: Aspergillus nidulans ; Mutation induction ; Mutagen dose ; Mutant yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Since the yield of mutants per surviving cell increases in general with increasing dose of mutagen, it has often been concluded in the literature that it is the most efficient to apply high mutagen doses so that most spores are killed. As high doses of mutagen produce chromosome rearrangements and unnoticed mutations which disturb the genetic background, the relationship between mutant frequency and survival was analyzed with Aspergillus nidulans as a model. It is shown that for different types of mutants the highest mutant yield is obtained at low mutagen doses (20–50% survival). Mutant frequency increases with increasing dose of mutagen but levels off and even decreases at higher dosages. There is no simple linear relationship between mutant frequency and the logarithm of the mutagen dose or the logarithm of the surviving fraction. If appropriate enrichment procedures are also available auxotrophic mutants can best be isolated at low doses of mutagen. Taking into account the disturbance of the genetic background, mutation induction should be done preferentially at a survival level of at least 70%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434826
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger (1988)
    Springer
    Current genetics 14 (1988), S. 437-443 
    Details
    ISSN: 1432-0983
    Keywords: Aspergillus niger ; Genetic analysis ; Genetic markers ; Linkage groups ; Master strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A start has been made on establishing a collection of Aspergillus niger colour and auxotrophic mutants with an isogenic background for use as a source of genetic markers. All strains have short conidiophores (cspAl ), which makes them easy to handle on test plates. Genetic markers were combined stepwise by somatic recombination. Somatic diploids were obtained at frequencies of 10−6-10−5 with conidiospores collected from a heterokaryon. The haploidization of heterozygous diploids was induced by benomyl. For unlinked markers, the frequency of recombinants varied from 35%–65%. Low frequencies of recombinants were found between markers on a same chromosome, but this was sometimes disturbed by mitotic crossing-over during an early stage of the diploid. Master strains were constructed having markers for six linkage groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00521266
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    Paper (German National Licenses)
    Fulltext
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