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  • Articles: DFG German National Licenses  (22)
  • Engineering General  (11)
  • Life Sciences  (6)
  • Avena  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Protein patterns in the oat coleoptile as influenced by auxin and by protein turnover (1980)
    Springer
    Planta 148 (1980), S. 429-436 
    Details
    ISSN: 1432-2048
    Keywords: Auxin action ; Avena ; Coleoptile ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synthesis of growth-limiting proteins (GLP) is required for continued auxin-induced elongation of oat (Avena sativa L.) coleoptiles. In order to determine whether GLP synthesis is dependent or independent of auxin, a double-labeling ratio technique, coupled with disc-gel electrophoresis, has been used to assess the effect of auxin on the pattern of protein synthesis. Sections were peeled to enhance amino-acid uptake; proteins were labeled with [14C]- or [3H] leucine in the presence or absence of indole-3-acetic acid for 40 min to 6 h, and were separated into soluble, membrane-associated, and wall-associated fractions. Regardless of the conditions used, or the protein fraction examined, no changes in response to auxin were detected in the pattern of protein synthesis. In order to escape detection by this technique an auxin-induced protein would have to comprise less than 0.75% of the total newly synthesized protein. Thus the synthesis of GLP appears to be independent of auxin. The same technique has been used to follow protein turnover. During the chase, proteins are initially degraded at an average rate of 8% h−1, and some protein bands showed as much as 14% h−1 degradation. No protein was detected which had a turnover rate as rapid as the GLP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395310
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The contribution of tonoplast and plasma membrane to the electrical properties of a higher-plant cell (1978)
    Springer
    Planta 143 (1978), S. 261-265 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Cells (electric propeties) ; Electrical parameters ; Fusicoccin ; Plasmalemma ; Tonoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cytoplasm of subepidermal parenchyma cells of Avena sativa L. coleoptiles was collected at one end of the cell by centrifugation. The electrical properties of both plasmalemma and tonoplast were then examined with microelectrodes inserted into both cytoplasm and vacuole of the same cell. The input resistance of the cytoplasm measured with either electrode was 7.5±0.8 MΩ while that of the vacuole measured with the single vacuolar electrode and a bridge circuit was 29.2±3.1 MΩ. The latter value was not significantly different from that of control, uncentrifuged cells. The resistance of the tonoplast is therefore several times larger than the input resistance of the cytoplasm, but the specific resistance of the plasma membrane cannot be calculated without knowledge of the extent and pattern of intercellular coupling. Electrical coupling of the cytoplasms of adjacent cells was observed in only two out of eight experiments. The mean potential of the vacuoles,-77.8±6.4 mV, was not significantly different from that of the cytoplasm; however, all the available evidence indicates that variable tip potentials in impaled cells made absolute determination of the membrane potential uncertain. In fusicoccin, the cells hyperpolarized by 20 mV within 10 min. This reponse occurred entirely at the plasmalemma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391996
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Protein synthesis and auxin-induced growth: Inhibitor studies (1979)
    Springer
    Planta 145 (1979), S. 437-442 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Avena ; Cell elongation ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have compared the effects of cycloheximide (CHI) and two other rapid and effective inhibitors of protein synthesis, pactamycin and 2-(4-methyl-2,6-dinitroanilino)-N-methyl proprionamide (MDMP), on protein synthesis, respiration, auxin-induced growth and H+-excreation of Avena sativa L. coleoptiles. All three compounds inhibit protein synthesis without affecting respiration. The effectiveness of the inhibitors against H+-excretion and growth correlates with their ability to inhibit protein synthesis. Both CHI and MDMP inhibit auxin-induced H+-excretion after a latent period of 5–8 min, and inhibit growth after a 8–10-min lag. These results support the idea that continued protein synthesis is required in the initial stages of the growth-promoting action of auxin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380097
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Protein patterns in the oat coleoptile as influenced by auxin and by protein turnover (1980)
    Springer
    Planta 148 (1980), S. 429-436 
    Details
    ISSN: 1432-2048
    Keywords: Auxin action ; Avena ; Coleoptile ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synthesis of growth-limiting proteins (GLP) is required for continued auxin-induced elongation of oat (Avena sativa L.) coleoptiles. In order to determine whether GLP synthesis is dependent or independent of auxin, a double-labeling ratio technique, coupled with disc-gel electrophoresis, has been used to assess the effect of auxin on the pattern of protein synthesis. Sections were peeled to enhance amino-acid uptake; proteins were labeled with [14C]- or [3H] leucine in the presence or absence of indole-3-acetic acid for 40 min to 6 h, and were separated into soluble, membrane-associated, and wall-associated fractions. Regardless of the conditions used, or the protein fraction examined, no changes in response to auxin were detected in the pattern of protein synthesis. In order to escape detection by this technique an auxin-induced protein would have to comprise less than 0.75% of the total newly synthesized protein. Thus the synthesis of GLP appears to be independent of auxin. The same technique has been used to follow protein turnover. During the chase, proteins are initially degraded at an average rate of 8% h−1, and some protein bands showed as much as 14% h−1 degradation. No protein was detected which had a turnover rate as rapid as the GLP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552655
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of fusicoccin on the activity of a key pH-stat enzyme, PEP-carboxylase (1978)
    Springer
    Planta 139 (1978), S. 43-45 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Cell elongation ; Fusicoccin ; Malate synthesis ; PEP carboxylase ; pH stat ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phytotoxin fusicoccin (FC) causes rapid synthesis of malate in coleoptile tissues, presumably via phosphoenolpyruvate (PEP) carboxylase coupled with malate dehydrogenase. The possibility that FC directly affects PEP carboxylase in Avena sativa L. and Zea mays L. coleoptiles was studied and rejected. The activity of this enzyme is unaffected by FC whether FC is added in vitro or a pretreatment to the live material. FC does not change the sensitivity of the enzyme to bicarbonate or malate. The activity of FC, instead, appears to be indirect. The pH sensitivity of PEP carboxylase is such that its activity, and thus the rate of malate synthesis, may be enhanced by an increase in cytoplasmic pH accompanying FC-induced H+ excretion. Since the enzyme is also particularily sensitive to bicarbonate levels, malate synthesis may also be enhanced by FC-induced uptake or generation of CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390808
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Reduction of the error in eccentric beam modelling (1980)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 575-582 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The usual linear shape function for approximating axial displacement in beam finite elements used as eccentric stiffeners leads to an inconsistency which can result in large errors in the deflection of plates or composite beams. The introduction of an additional degree-of-freedom for these elements circumvents this problem.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620150408
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  • 7
    Electronic Resource
    Electronic Resource
    Dynamic aspects of the error in eccentric beam modelling (1980)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 1447-1455 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The usual linear shape function for approximating axial displacements in beam finite elements used as eccentric stiffeners or as portions of composite cross-sections, leads to an error in the static deflection. A modified element reduces this error. The behaviour of these two elements under free vibration is examined. The linear element is shown to be in considerable error in the dynamic case also.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620151003
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    An initial-value method for integral operators: III - Refinement of complex eigenvalues by Newton's method (1975)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 9 (1975), S. 944-948 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620090414
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  • 9
    Electronic Resource
    Electronic Resource
    Development and evaluation of two non-linear shell elements (1980)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 16 (1980), S. 65-80 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Two plate and shell displacement elements are developed for use in large deflection non-linear analysis. The elements are of the 'stability' type, in which non-linear strains are included, with their values optimized by added higher order membrane functions and special types of elemental level constraints. The paper summarizes formulation and computational procedures, and discusses numerical results in detail. Conclusions are given regarding the effectiveness of the elements for solving both linear and non-linear shell analysis problems.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620160107
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  • 10
    Electronic Resource
    Electronic Resource
    Vibrations of twisted cantilever plates - a comparison of theoretical results (1985)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 21 (1985), S. 1365-1380 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Previously published literature shows widely different results for the free vibration frequencies of twisted cantilever plates. Inasmuch as it is important to know the vibration characteristics of turbomachinery blades, which may have considerable twist, it would be desirable to have a comprehensive, definitive set of results, and to establish which of the numerous theoretical methods available can adequately analyse such problems. For this purpose, a joint government/industry/university research study was organized. Numerical results were obtained for a set of 20 different twisted plates having various aspect ratios, thickness ratios and pretwist angles. Nineteen distinct theoretical methods were employed, 15 using finite elements, two using shell theory, and two using beam theory. Although some of the best-known computational procedures (especially finite element codes) were used by analysts with great experience, the numerical results obtained showed considerable disagreement. The present paper describes the analytical methods used and exhibits samples of the type of results obtained.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620210802
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    Paper (German National Licenses)
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