ISSN:
1040-0397
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Reduced nicotinamide adenine dinucleotide (NADH) has been determined at submicromolar levels (the detection limit was 0.05 μmol/L) by using graphitized and glassy carbon electrodes obtained from different sources. Preliminary studies compared the sensitivity, stability, and reproducibility of such sensors during NADH measurements in linear-sweep voltammetry as well as at constant applied potential. High purity graphite carbon from Ringsdorff has been found to be the most suitable for NADH oxidation. To regenerate the electrodes after each voltammetric scan, washing with a large volume of distilled water under stirring conditions is required. When the sensitivity of the electrode decreases drastically, the sensor surface must be ground and polished with alumina. The nature of the carbon surface has a pronounced effect on the NADH response.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140010303
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