ISSN:
1432-0983
Keywords:
Prokaryotic promoters
;
Chloroplast DNA replication origin
;
Chlamydomonas reinhardtii
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pK01 was used to screen for the presence of any prokaryotic promoter within the cloned fragment. The insertion of 2 AluI fragments yielded galK+ colonies. Sequence analyses of these Alul inserts revealed prokaryotic promoter consensus regions. Cloning into pKOTWI and subsequent DNA sequencing were used to determine the promoter-active orientation of each insert. Two back-to-back prokaryotic promoters were mapped on a 79 by Alul fragment located within the displacement loop region.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00418528
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