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  • Articles: DFG German National Licenses  (6)
  • Plasma  (3)
  • Chromosome substitutions  (2)
  • Organophosphate  (2)
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  • Articles: DFG German National Licenses  (6)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Studies of rat alkaline phosphatase (1978)
    Springer
    Archives of toxicology 39 (1978), S. 267-287 
    Details
    ISSN: 1432-0738
    Keywords: Rat ; Plasma ; Alkaline phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes the application of a test system which applies different substrates, inhibitors and acrylamide electrophoresis to the study of the alkaline phosphatase activity of rats. The system, which was specifically devised to characterise the alkaline phosphatase activity of small quantities of plasma from rats in toxicological studies, was also used to examine the plasma from rats under several physiological conditions, e.g. time of day, age, diet, etc. These physiological factors can influence the measurement of plasma alkaline phosphatase activity and should be taken into account when designing toxicological studies. Different experimental regimes were used to produce increases in liver, bone and intestinal alkaline phosphatase isoenzymes in the plasma of rats and these were reflected in the characterisations by the system. Although much remains unknown, a few concepts of rat plasma alkaline phosphatase activity emerged under our conditions. There is a ‘tidal’ intestinal component which arises from feeding and is approximately 60% of the total activity of the fed rat. In the fasted rat this ‘tidal’ component has largely disappeared and the ‘core’ activity which remains is composed of approximately equal proportions of bone isoenzyme and another as yet ill-defined component. The liver isoenzyme which appears in the plasma of the cholestatic rat appears to be either a very minor component or absent from the plasma of our normal CFE derived rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296387
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Methods for the estimation of acetylcholinesterase activity in the plasma and brain of laboratory animals given carbamates or organophosphorus compounds (1971)
    Springer
    Archives of toxicology 27 (1971), S. 292-310 
    Details
    ISSN: 1432-0738
    Keywords: Cholinesterase ; Plasma ; Brain ; Carbamate ; Organophosphorus Compounds ; Cholinesterase ; Plasma ; Gehirn ; Carbamate ; Organophosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Dieser Report beschreibt die Modifikationen, die notwendig sind, um die pH-Stat und Ellman-Methode für die Bestimmung der Cholinesteraseaktivität im Plasma und Gehirn der verschiedenen Laboratoriumstierarten anzupassen. Beide Methoden wurden durch Untersuchungen bewertet, bei denen den Tieren cholinesterasehemmende Stoffe, Carbamate und organische Phosphorverbindungen, gegeben worden waren.
    Notes: Abstract This report describes the modifications necessary to adapt the pH Stat and Ellman methods for the estimation of cholinesterase activity in the plasma and brain of various species of laboratory animals. Both methods were evaluated in studies in which cholinesterase inhibitors, carbamates and organophosphorus compounds, were given to the animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315051
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Studies of rat alkaline phosphatase (1978)
    Springer
    Archives of toxicology 39 (1978), S. 249-266 
    Details
    ISSN: 1432-0738
    Keywords: Rat ; Alkaline phosphatase ; Plasma ; Organs ; Toxicity testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A manual system of various estimations of rat plasma alkaline phosphatase activity has been devised for small volumes of plasma which uses different inhibitors, compares the utilisation of two substrates and includes acrylamide gel electrophoresis. The different inhibitors etc. allow a degree of discrimination between alkaline phosphatase extracts of rat organs. The properties of isoenzymes, e.g. intestinal alkaline phosphatase, differ depending upon the environment in which they are studied. In conjunction, if necessary, with the methods described for the estimation of liver and intestinal alkaline phosphatase activity, it is hoped to use the system to discriminate between the isoenzymes present in the plasma alkaline phosphatase of rats in toxicological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296386
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Methods for the estimation of acetylcholinesterase activity in the erythrocytes of laboratory animals given carbamates or organophosphorus compounds (1974)
    Springer
    Archives of toxicology 31 (1974), S. 197-216 
    Details
    ISSN: 1432-0738
    Keywords: Acetylcholinesterase ; Erythrocytes ; Carbamate ; Organophosphorus Compounds ; Cholinesterase ; Erythrocyten ; Carbamate ; Organophosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Dieser Bericht beschreibt die Modifikationen, die zur Anpassung der pH Stat und einer Δ pH-Methode für die Bestimmung der Acetylcholinesteraseaktivität in den Erythrocyten verschiedener Laboratoriumstierarten notwendig sind. Beide Methoden werden für Untersuchungen eingesetzt, bei denen den Tieren Cholinesteraseinhibitoren, Carbamate und Organophosphate, verabreicht worden waren.
    Notes: Abstract This report describes the modifications necessary to adapt the pH stat and a Δ pH method for the estimation of acetylcholinesterase activity in the erythrocytes of various species of laboratory animals. Both methods were evaluated in studies in which cholinesterase inhibitors, carbamates and Organophosphorus compounds, were given to the animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00311053
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Monosomic and double monosomic substitutions of Hordeum bulbosum L. chromosomes into H. vulgare L. (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 466-472 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare L. ; Hordeum bulbosum L. ; Interspecific hybrids ; Chromosome substitutions ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One of the aims of the interspecific crossing programme between Hordeum vulgare L. and H. bulbosum L. has been to introgress desirable genes into barley from the wild species. However, despite their close taxonomic relationship there have been few reports of achieving this objective using amphidiploid hybrids. In order to broaden the range of available hybrids, partially fertile triploids between H. vulgare (2n = 2x = 14) and H. bulbosum (2n = 4x = 28) were developed and crossed with H. vulgare as female parent. From 580 progeny which were screened, eight putative single monosomic chromosome substitution lines and one double monosomic substitution were identified by cytological analysis. These involved the substitution of H. vulgare chromosome 1 (two lines), 6 (four lines), 6L (one line), 7 (one line) and 1 + 4 (one line) by their respective H. bulbosum homoeologues. The H. bulbosum chromosome was frequently eliminated during plant development, but it was observed regularly in pollen mother cells of two lines. However, pairing between the H. bulbosum chromosome and its H. vulgare homoeologue was low. Several of the lines were more resistant than their H. vulgare parents to powdery mildew (Erysiphe graminis DC. f.sp. hordei Em. Marchai), net blotch (Drechslera teres Sacc.) and scald (Rhynchosporium secalis (Oudem.) Davis). Apart from their use in studying genome relationships, their value to plant breeders will depend on the ease of inducing translocations between the parental chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229508
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Characterisation of progeny from backcrosses of triploid hybrids between Hordeum vulgare L. (2x) and H. bulbosum L (4x) to H. vulgare (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 460-464 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Hordeum bulbosum ; Fertile triploids ; Gene introgression ; Chromosome substitutions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific hybridisations between Hordeum vulgare L. (cultivated barley) and H. bulbosum L. (bulbous barley grass) have been carried out to transfer desirable traits, such as disease resistance, from the wild species into barley. In this paper we report the results of an extensive backcrossing programme of triploid hybrids (H. vulgare 2x x H. bulbosum 4x) to two cultivars of H. vulgare. Progenies were characterised cytologically and by restriction fragment length polymorphism analysis and comprised (1) haploid and diploid H. vulgare plants, (2) hybrids and aneuploids, (3) single and double monosomic substitutions of H. bulbosum chromosomes into H. vulgare and (4) chromosomal rearrangements and recombinants. Five out of the seven possible single monosomic chromosome substitutions have now been identified amongst backcross progeny and will be valuable for directed gene introgression and genome homoeology studies. The presence amongst progeny of 1 plant with an H. vulgare-H. bulbosum translocated chromosome and one recombinant indicates the value of fertile triploid hybrids for interspecific gene introgression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223661
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    Paper (German National Licenses)
    Fulltext
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