ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— The Enzyme 2′, 3′-cyclic AMP 3′-phosphohydrolase (CNP) is regarded as a marker for myelin (KURI- HARA and MANDEL, 1970) on the basis of its regional and subcellular distribution (Kurihara and Tsukada, 1967), its ontogenetic characteristics (KURIHARA and TSUKADA, 1968), and its behaviour in two strains of myelin-deficient mutant mice (Kurihara, Nussbaum and Mandel, 1969). However we have isolated highly-purified preparations of neuronal plasma membrane from rat brain synaptosomes which contain this enzyme activity (Morgan, Wolfe, Mandel and Gombos, 1971). Two explanations of this finding are possible. The activity could be due to the presence of myelin, but this explanation is ruled out by electron microscopy and by the low level of cerebrosides in the synaptosomal plasma membrane preparations. Myelin is extremely rich in cerebrosides (norton and Autilio, 1966). The second possibility is that the enzyme, 2′, 3′-cyclic AMP 3′-phosphohyrolase, may also be found in the glial cells from which myelin is derived (Bunge, Bunge and Pappas, 1962). To test our hypothesis that 2′, 3′-cyclic AMP 3′-phosphohydrolase is not a specific marker for myelin, but is also found, in glial cells, we have examined a tumoral glial cell line maintained in myelin-free tissue culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1972.tb01401.x
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