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  • Articles: DFG German National Licenses  (3)
  • Dog  (2)
  • Culture  (1)
  • 1
    Electronic Resource
    Electronic Resource
    NADPH-diaphorase and nitric oxide synthase in the canine superior cervical ganglion (1995)
    Springer
    Cell & tissue research 279 (1995), S. 629-631 
    Details
    ISSN: 1432-0878
    Keywords: NADPH-diaphorase ; Nitric oxide synthase ; Superior cervical ganglion ; Dog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract By means of NADPH-diaphorase (NADPH-d) histochemistry and nitric oxide synthase (NOS) immunohistochemistry, we demonstrate that considerable numbers of NADPH-d-positive neurons are distributed throughout the canine superior cervical ganglion (SCG). These neurons also show NOS immunoreactivity. This finding indicates that NADPH-d histochemistry, a simple and reliable technique, can be used as a reliable marker of NOS activity in the sympathetic innervation of canine head and neck. The present findings suggest that the participation of nitric oxide in the SCG differs greatly between species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318175
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    NADPH-diaphorase and nitric oxide synthase in the canine superior cervical ganglion (1995)
    Springer
    Cell & tissue research 279 (1995), S. 629-631 
    Details
    ISSN: 1432-0878
    Keywords: Key words: NADPH-diaphorase ; Nitric oxide synthase ; Superior cervical ganglion ; Dog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. By means of NADPH-diaphorase (NADPH-d) histochemistry and nitric oxide synthase (NOS) immunohistochemistry, we demonstrate that considerable numbers of NADPH-d-positive neurons are distributed throughout the canine superior cervical ganglion (SCG). These neurons also show NOS immunoreactivity. This finding indicates that NADPH-d histochemistry, a simple and reliable technique, can be used as a reliable marker of NOS activity in the sympathetic innervation of canine head and neck. The present findings suggest that the participation of nitric oxide in the SCG differs greatly between species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050322
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Isolation, growth, and characteristics of human ovarian surface epithelium (1994)
    Springer
    Medical molecular morphology 27 (1994), S. 264-267 
    Details
    ISSN: 1860-1499
    Keywords: Human ovary ; Culture ; Growth ; Ovarian surface epithelium ; Characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To isolate human ovarian surface epithelium (OSE) and studied its characteristicsin vitro, we used the scraping method with an enzymatic procedure. After incubation of the ovary in collagenase type 1 solution, the surface cells were removed by gentle scraping with surgical blade. Cells obtained as a cluster after unit gravity sedimentation with 5% bovine serum albumin in medium 199 were cultured in medium 199 containing 15% fetal bovine serum. The viable cell number in a single ovary was 0.1 to 2.7×106, and the cell population doubling time was between 7 to 10 days. Confluent monolayers were formed after 13–20 days and subcultured one to three times. The monolayers mostly had a cobblestone appearance, and fusiform or polygonal cells were also observed. By cytochemistry, immunocytochemistry and scanning and transmission electron microscopy, the cells in short-term culture were shown to have characteristics of mesothelial OSE cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02349671
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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