ISSN:
1432-2307
Keywords:
Alloxan
;
Culture
;
Electron microscopy
;
6-Hydroxydopamine
;
Pancreaticβ-cells
;
Stereology
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Stereological techniques on electron microscopy micrographs were used to evaluate the morphological changes of cultured isletβ cells that had been exposed to alloxan or 6-hydroxydopamine. Trypan Blue exclusion by cells cultured for 3 days indicated that the cells were 100% viable. Electron microscopy revealed that nearly all of the surviving cultured cells wereβ cells. Exposure to 5 mmol/l alloxan or 1–5 mmol/l 6-hydroxydopamine for 10 or 30 min caused a general swelling of the cultured cells with a concomitant swelling of mitochondria and nuclei. The size of the secretory granules was not affected by the drugs. Only 3–10% of the cells excluded Trypan Blue after exposure to 5 mmol/l alloxan or 6-hydroxydopamine. The data conform with the hypothesis that a primary action of alloxan and 6-hydroxydopamine is at the plasma membrane level ofβ cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00704248
Permalink
