ISSN:
1432-0533
Keywords:
S-100 Protein
;
Immunoelectron microscopy
;
Cultured rat glioma cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Immunoelectron microscopic localization of the nervous system specific protein S-100 in the cultured rat glioma cells was successfully conducted by an unique immunocytochemical technique using peroxidase-labeled antigen binding Fab' fragments. The intensely electron dense reaction product for S-100 protein was localized mainly at ribosome granules associated with endoplasmic reticulum membranes and at free ribosome granules. S-100 protein was also associated to some extent with the cytoplasmic and nuclear membranes. A positive reaction was localized at the nuclear pores as if it were being prevented from entering into the nucleus. No activity was found in the nucleoplasm except for a slightly positive reaction product associated with nucleolus. The possible role for S-100 protein in neural cells was discussed in relation to the nuclear acidic proteins involved in genomic regulation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00691485
Permalink
