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  • Articles: DFG German National Licenses  (4)
  • Cytoplasmic male sterility  (2)
  • Diplotaxis catholica  (2)
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  • Articles: DFG German National Licenses  (4)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Diplotaxis catholica + Brassica juncea somatic hybrids: molecular and cytogenetic characterization (1995)
    Springer
    Plant cell reports 14 (1995), S. 593-597 
    Details
    ISSN: 1432-203X
    Keywords: Brassica juncea ; Diplotaxis catholica ; Somatic hybrids ; Organelle constitution ; Mitochondrial DNA recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric somatic hybrids Diplotaxis catholica (2n=18) + Brassica juncea (2n=36) were produced by fusing mesophyll protoplasts of the former and hypocotyl protoplasts of the latter using polyethylene glycol. Out of 52 somatic embryos, 24 produced plants of intermediate morphology. Cytological analysis of 16 plants indicated that 15 were symmetric hybrids carrying 54 chromosomes, the sum of the parental chromosome numbers. One hybrid was asymmetric with 45 chromosomes. Nuclear hybridity of five putative hybrids was confirmed by the Southern hybridization pattern of full length 18s-25s wheat nuclear rDNA probe which revealed the presence of Hind III fragments characteristic of both the parental species. The hybridization pattern of mitochondria specific gene probe cox I indicated that three of the hybrids carried B. juncea mitochondria and one carried mitochondria of D. catholica. Presence of novel 3.5 kb Hind III and 4.8 kb Bgl II fragments suggested the occurrence of mtDNA recombination in one of the hybrids. The hybrids were pollen sterile. However, seeds were obtained from most of the hybrids by back crossing with B. juncea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231945
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Male sterility caused by cytoplasm of Brassica oxyrrhina in B. campestris and B. juncea (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 285-287 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility ; Brassica oxyrrhina ; B. campestris ; B. juncea ; Alien gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Synthetic alloploid Brassica oxyrrhina (2n = 18, OO) x B. campestris (2n = 20, AA) was repeatedly backcrossed with B. campestris to place B. campestris nucleus in the cytoplasm of B. oxyrrhina. Alloplasmic plants, obtained in BC5 generation, were stably male sterile but mildly chlorotic during initial development. Synthetic alloploid B. oxyrrhina-campestris was also hybridized with B. juncea to transfer B. oxyrrhina cytoplasm. Segregation for green and chlorotic plants was observed in BC1 and BC2 generations. By selection, however, normal green male sterile B. juncea was obtained in BC3. Pollen abortion in both B. campestris and B. juncea is post-meiotic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225965
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Chloroplast substitution overcomes leaf chlorosis in a Moricandia arvensis-based cytoplasmic male sterile Brassica juncea (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1179-1182 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica juncea ; Moricandia arvensis ; Cytoplasmic male sterility ; Chlorosis correction ; Cytoplasmic hybrids ; Chloroplast substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A male sterile Brassica juncea line based on Moricandia arvensis cytoplasm was developed previously by backcrossing the somatic hybrid M. arvensis+B. juncea, and the gene for restoring fertility was introgressed. The CMS line is very severely chlorotic because of the presence of alien chloroplasts and flowering is delayed by 30–40 days, making it unsuitable for the exploitation of heterosis. We have resorted to another cycle of protoplast fusion between green fertile B. juncea and chlorotic male sterile B. juncea, and developed green male-sterile plants. Molecular analysis revealed that in green male-sterile plants chloroplasts of M. arvensis origin were substituted by those from B. juncea, giving rise to intergeneric cytoplasmic hybrids with mitochondria of M. arvensis origin. With the development of dark-green male-sterile plants, the CMS fertility restoration system is suitable for the production of hybrid mustard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051007
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Random chloroplast segregation and mitochondrial genome recombination in somatic hybrid plants of Diplotaxis catholica+Brassica juncea (1998)
    Springer
    Plant cell reports 17 (1998), S. 814-818 
    Details
    ISSN: 1432-203X
    Keywords: Key words Random chloroplast segregation ; Mitochondrial genome recombination ; Diplotaxis catholica ; Brassica juncea ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Detailed molecular analysis of the somatic hybrid plants of Diplotaxis catholica+B. juncea indicated random chloroplast segregation. One of the five hybrid plants analyzed derived its chloroplasts from D. catholica and two hybrids had chloroplasts of B. juncea origin. Two hybrid plants maintained mixed population of chloroplasts. The mitochondrial (mt) genomes of the fusion partners had undergone recombinations. Occurrence of fragments specific to both the parents in HindIII digestion followed by atp 9 probing, as in hybrid DJ5, provided evidence for intergenomic mitochondrial recombination between D. catholica and B. juncea. Similar mt genome organization in two hybrids (DJ3 and DJ6) suggested that intergenomic recombination may be preferred at specific sites. Hybrid DJ1 had about 70% similarity to D. catholica in mt genome organization. mt genomes of hybrids DJ2, 3, 5, and 6 differed from B. juncea by 14.3–28%. The significance of these novel mt genome organizations in developing novel male sterility systems is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050489
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    Paper (German National Licenses)
    Fulltext
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