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  • Articles: DFG German National Licenses  (3)
  • DNA ligase  (1)
  • Insulin-like growth factor  (1)
  • Life and Medical Sciences  (1)
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  • Articles: DFG German National Licenses  (3)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    In vitro approach to the control of spermatogonia proliferation in the trout
    Amsterdam : Elsevier
    Molecular and Cellular Endocrinology 102 (1994), S. 141-150 
    Details
    ISSN: 0303-7207
    Keywords: (Sertoli cell) ; (Spermatogonia) ; (Trout) ; Gonadotropin ; Growth hormone ; Insulin-like growth factor ; Steroid
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0303-7207(94)90107-4
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Expression of DNA ligase genes by ram spermatid nuclei and RNA in amphibian eggs (1986)
    Springer
    Development genes and evolution 195 (1986), S. 186-192 
    Details
    ISSN: 1432-041X
    Keywords: DNA ligase ; Gene activity ; Nuclear transplantation ; Ram spermatids ; Axolotl egg
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary During animal development and gametogenesis two DNA ligases are found and successively expressed. In this study the two DNA ligases present in the axolotl egg and the two ligases present during ram sperm cell maturation were distinguished by biochemical and immunological methods. The expression of the genes for the heavy and light ram DNA ligases has been studied using transplantation of spermatid and sperm nuclei in axolotl eggs. We found that ram DNA ligases were expressed in axolotl egg cytoplasm. The exclusion phenomenon between the heavy and light form of DNA ligase is species-specific and involves a cytoplasmic mediator. In the transplanted ram germ cell nuclei the heavy ram DNA ligase expression was found to be sensitive to inhibitors of transcription while the light one was not. When mRNA was used, no exclusion process was observed and both the heavy and light enzyme expression were sensitive to cycloheximide and not to aamanitin. These results are discussed in terms of the possible stability of the gene-regulated state following nuclear transfer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02439437
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of protamine in the spermatids of the ram (1983)
    New York, NY : Wiley-Blackwell
    Gamete Research 8 (1983), S. 21-28 
    Details
    ISSN: 0148-7280
    Keywords: ram ; spermiogenesis ; nuclear composition ; protamine immunocytochemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Pure ram protamine isolated from epididymal spermatozoa was used to raise antisera in castrated rabbits. The antibodies were visualized in the electron microscope using the method of Moriarty and Halmi [1972] with either peroxidase or coupling with colloidal gold. The gold gave better contrast but lower afinity than the peroxidase method. With the use of fixation according to Thiery and Rambourg [1976] and thick sections treated with hydrogen peroxide, it was possible to detect the protamine in the cytoplasm near the flagellum and the chromatoid body of step 12 spermatids. It was concluded that protamine enters the nucleus and concentrates at that step in the ram.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120080104
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    Paper (German National Licenses)
    Fulltext
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