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  • Articles: DFG German National Licenses  (2)
  • DNAase restriction  (1)
  • Protoplast co-culture  (1)
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  • Articles: DFG German National Licenses  (2)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybridization of an atrazine resistant biotype of Solanum nigrum with Solanum tuberosum (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 131-134 
    Details
    ISSN: 1432-2242
    Keywords: Plastome segregation ; DNAase restriction ; Protoplast fusion-triazine resistance ; Solanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Representative regenerated clonal plants from protoplast fusion of Solanum tuberosum L. and an atrazine resistant biotype of S. nigrum L. were studied to ascertain which plastomes each clone contained. DNA was isolated from fractionated chloroplasts, restricted with DNAases XHO-1, BGL-1, PVU-2 and BAM-H1, and the fragments separated by agarose gel electrophoresis for comparison. No difference could be found between resistant and susceptible biotypes of S. nigrum with all four enzymes. XHO-1, BGL-1, BAM-H1 differentiated between S. nigrum and S. tuberosum. All atrazine resistant regenerants, despite plant morphology, had the plastid DNA pattern of S. nigrum while all sensitive ones resembled S. tuberosum, even the subclone 38S having a S. nigrum morphology and chromosome number.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317018
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Plant cell graft chimeras obtained by co-culture of isolated protoplasts (1987)
    Springer
    Protoplasma 141 (1987), S. 64-73 
    Details
    ISSN: 1615-6102
    Keywords: Cell grafting ; Heterospecific plasmodesmata ; Plant chimeras ; Protoplast co-culture ; Solanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Heterospecific chimeralSolanum nigrum (+)Solanum tuberosum plants were obtained by cell grafting in protoplast co-cultures. Periclinal, sectorial, and mericlinal chimeras have been identified by various morphological and cytological characteristics. Morphogenesis predominantly began in periclinal chimeral organization. Cells of different species have been found to be interconnected by secondary plasmodesmata. Plantlets of all chimeral lines were grown to flowering under tissue culture conditions and some also in the greenhouse. Aspects of organogenesis and interspecific cooperation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276789
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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