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  • Articles: DFG German National Licenses  (2)
  • Diabetes  (1)
  • Microtubules  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Biochemical and ultrastructural changes in A and B cells of the islets of Langerhans of mice infected with EMC virus (1979)
    Springer
    Diabetologia 17 (1979), S. 51-57 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes ; EMC virus ; DBA2 mice ; islets of Langerhans ; ultrastructure ; insulin ; glucagon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Infection of DBA2 mice with the M strain of EMC virus was used to study the effects of virusinduced diabetes on the A and B cells of the islets of Langerhans. A transient hypoglycaemia was seen in 48% of mice 2–3 days after infection and probably resulted from increased serum insulin concentrations together with inhibition of glucagon secretion at that time. Islets from hypoglycaemic mice showed no significant alterations from control level in basal or fluoride-stimulated adenylate cyclase activity. Overall, 70% of infected mice became hyperglycaemic with a maximum incidence 6 days after infection. Hyperglycaemia was accompanied by a dramatic reduction in the total pancreatic insulin content and in insulin secretory responses to glucose and theophylline, while A-cell structure and function appeared relatively unaffected in diabetic animals. Basal adenylate cyclase activity was increased in hyperglycaemic mice at 7 days after infection, while fluoride-stimulated adenylate cyclase activity was normal throughout the course of infection. Ultrastructural alterations were observed in a small proportion of B cells from two days after infection and included abnormalities of mitochondrial structure and increased electron opacity of the cytoplasm of affected cells, which subsequently led to complete necrosis. The results suggest that EMC virus specifically affects the B cells of the islets and that disturbances of A cell function may be secondary to B cell damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01222978
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Role of microtubules in the intracellular transport of growth hormone (1978)
    Springer
    Cell & tissue research 190 (1978), S. 163-171 
    Details
    ISSN: 1432-0878
    Keywords: Growth hormone ; Somatotrophs ; Microtubules ; Colchicine, vinblastine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pulse-chase experiments utilising(3H)leucine have been used to study the effects of colchicine and vinblastine on intracellular transport and secretion of newly synthesised growth hormone from rat anterior pituitary fragments. Growth hormone was isolated from medium and fragments by polyacrylamide gel electrophoresis. When colchicine or vinblastine, which disrupt microtubules, were added immediately after pulse labelling, inhibition of the subsequent secretion of newly synthesised growth hormone was detected throughout the succeeding 5 h. Similar inhibition was seen if the drugs were added after a 1 h delay. However, if colchicine or vinblastine were added only after a 2 h chase incubation, then no significant effect on subsequent release of labelled growth hormone was seen. The results suggest that these agents may inhibit the transport of newly formed growth hormone storage granules from the Golgi complex to the cytoplasmic pool. Microtubules do not appear to be involved in the mechanism of the final secretion of newly synthesised hormone by exocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210045
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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