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  • Articles: DFG German National Licenses  (2)
  • Endothelial cells  (1)
  • Key words Insulin-dependent diabetes  (1)
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  • Articles: DFG German National Licenses  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Methodology for retinal photography and assessment of diabetic retinopathy: the EURODIAB IDDM Complications Study (1995)
    Springer
    Diabetologia 38 (1995), S. 437-444 
    Details
    ISSN: 1432-0428
    Keywords: Key words Insulin-dependent diabetes ; diabetic retinopathy ; retinal photography ; grading scheme.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We present the methodology for 45° retinal photography and detail the development, application and validation of a new system of 45° field grading standards for the assessment of diabetic retinopathy. The systems were developed for the EURODIAB IDDM Complications Study, part of a European Community funded Concerted Action Programme into the epidemiology and prevention of diabetes (EURODIAB). Assessment of diabetic retinopathy was carried out centrally by a trained reader of colour retinal photographs using the newly-developed system. The system proved to be acceptably accurate, repeatable and relatively simple to apply. It compared well with the recognised ’gold standard' 7-field 30° stereo photography (assessed using a modified Airlie House classification scheme), against which the new system was validated in a series of 48 eyes. Selection was as a stratified random sample based on clinical retinopathy status: 5, no retinopathy; 25, non-proliferative retinopathy; 16, proliferative or photocoagulated; plus 2, eyes with potentially confounding lesions (vein occlusion). Simple presence of retinal lesions was correctly detected by both systems in 43 of the 48 eyes, giving 100 % agreement on detection. Both systems correctly identified the two known cases of confounding vein occlusion. In eyes with diabetic retinopathy (n = 41), when severity was expressed in three groups: mild background, moderate/severe background and proliferative/photocoagulated, at least one grader (out of five) using the new system matched the verified results in 38 out of 41 (93 %) eyes and three or more graders matched in 31 (76 %) eyes. Individually the five graders' 2-field allocations agreed well with the verified levels (median number of agreements 37, range 28–43). Repeatability was assessed by measures of within and between observer variation using randomly selected samples of 10 % (n = 252 eyes) and 5 % (n = 123 eyes) of the main study, respectively, expressed as a resultant kappa value for chance-corrected proportional agreement. Within observer assessment yielded a kappa of 0.85 and between observers a value of 0.83; indicating very good agreement for both measures. The method is particularly useful for large epidemiological studies, in which participating centres have a limited experience in retinal photography. [Diabetologia (1995) 38: 437–444]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410281
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A study of the effects of human blood derivatives and individual growth factors on [3H]thymidine uptake in bovine retinal pericytes and endothelial cells (1993)
    Springer
    Acta diabetologica 30 (1993), S. 207-213 
    Details
    ISSN: 1432-5233
    Keywords: Diabetes mellitus ; Diabetic retinopathy ; Endothelial cells ; Growth factors ; Pericytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pericytes disappear early, selectively and specifically from retinal capillaries in diabetic microangiopathy, but little is known of their growth and turnover in health and disease. We have studied the effects of human blood derivatives and of a panel of individual growth factors on [3H]thymidine incorporation in bovine retinal pericytes and endothelial cells. Human serum and platelet-rich plasma stimulated incorporation of the nucleotide in a dose-dependent manner in both cell types, and did so more potently than platelet-free plasma. Consistent and significant stimulation of DNA synthesis in pericytes was observed with basic fibroblast growth factor (ED50= 1.8×10−13 mol/l), acidic fibroblast growth factor (7.4× 10−12 mol/l), insulin-like growth factor 1 (8.6×10−10 mol/l), insulin (158 μU/ml) and endothelin-1 (6.1×10−10 mol/l). Transforming growth factor β1 inhibited DNA synthesis (ID50=3.6×10−10 mol/l) and so did heparin (1.4×10−6 mol/l) and low molecular weight heparin (2.9×10−6 mol/l). Retinal endothelial cells were stimulated by basic fibroblast growth factor (3.2×10−13 mol/l) and acidic fibroblast growth factor (1.3×10−9 mol/l), and inhibited by transforming growth factor β1, (1.6×10−12 mol/l). Neither cell type was stimulated by platelet-derived growth factor (A+B chain heterodimer), epidermal growth factor, growth hormone, or nerve growth factor (7S complex). The characteristics and active concentrations of the above growth factors suggest that none is solely responsible for the pericyte mitogenic activity of platelets, serum or plasma. Some, though, may play a role in the regulation of pericyte turnover through paracrine mechanisms which should be further investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569931
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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