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  • Articles: DFG German National Licenses  (3)
  • Islet transplantation  (1)
  • Key words Pancreas preservation  (1)
  • Temporary portocaval shunt.  (1)
  • 1
    ISSN: 1432-2277
    Keywords: Key words Pancreas preservation ; HTK-solution ; Segmental porcine pancreatic autotransplantation ; Delayed endocrine graft function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Delayed graft function (DEGF) remains an obscure phenomenon in organ transplantation. For the optimal washing of the compounds of the different organ flush solutions, adequate temperature and equilibrium of electrolytes have to be provided. A total of 29 landrace pigs weighing 37.3–5.4 kg were included in this study. According to the model, the left hemipancreas was perfused with Histidine-Tryptophan-Ketoglutarate (HTK)-solution and autotransplanted after 24 h (G1, n = 13) and 48 h (G2, n = 4) of cold storage (CS). Results were compared with grafts perfused with UW-solution and autotransplanted after 24 h (G3, n = 8) and 48 h (G4, n = 4) CS respectively. Daily measurements of glycemia, glucosuria, amylase and lipase were carried out. HTK perfusion resulted in an increase in wet weight of the grafts after 24 h and 48 h CS (P = 0.031 vs UW). Postoperative glycemia levels in pancreases flushed with HTK-solution were higher after 48 h than after 24 h CS until the 6th postoperative day, when the glycemia returned to normal range (P = 0.02), suggesting a delayed endocrine graft function. The mean IVGTT values attained after full function were comparable in G1 and in G3 (–1.22 ± 0.23 vs. –1.5 ± 0.65). The rises in serum amylase and lipase levels were more pronounced after 48 h CS in both HTK and UW groups, (P = n.s.). Appearance of interstitial and intracellular edema after CS and reperfusion did not influence the function.¶Conclusion: HTK-solution is suitable for 24 h pancreatic preservation in vivo; the perfusion requires at least 4 min for electrolyte equilibration. Long preservation time (48 h) resulted in a transitory DEGF.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-0385
    Keywords: Key words: Liver transplantation ; Temporary portocaval shunt. ; Schlüsselwörter: Lebertransplantation ; temporärer portocavaler Shunt.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Die laterolaterale Cavocavostomie zur hepatovenösen Rekonstruktion bei der Lebertransplantation wird vorgestellt, mit und ohne Anlage eines temporären portocavalen Shunts. Insgesamt 65 Lebertransplantationen wurden analysiert. Bei 49 Transplantationen wurde eine laterolaterale cavocavale Anastomose angelegt (Gruppe I). Bei 16 weiteren Patienten (Gruppe II) wurde ein temporärer portocavaler Shunt während der Hepatektomie durchgeführt. Der gemittelte arterielle Blutdruck betrug in mmHg für Gruppe I 128 ± 34, in Gruppe II 109 ± 32. Das Herzzeitvolumen (l/min) reduzierte sich während der anhepatischen Phase bei Gruppe I um 2,3 ± 1,9 bei Gruppe II um 1,2 ± 1,5 (p 〈 0,05). Der perioperative Blutverlust gemessen an der Anzahl Erythrocytenkonzentrate betrug 16,4 ± 15,8 in Gruppe I versus 1,2 ± 2,3 in Gruppe II (p 〈 0,04) an der Anzahl Einheiten Frischplasma 19,0 ± 14,7 vs. 3,7 ± 4,0 (p 〈 0,02). Der Verbleib auf der Intensivstation in Tagen, die Leberfunktionsteste sowie die renale Funktion und die Häufigkeit erforderlicher Reoperationen wegen Blutung waren nicht statistisch signifikant unterschiedlich zwischen den beiden Gruppen. Die 1-Jahres-Überlebensrate betrug 82,7 % bzw. 85,7 %. Schlußfolgerung: Die Anlage eines temporären portocavalen Shunts kann bei der Cava-erhaltenden Hepatektomie und Spender-zu-Empfänger-Cavocavostomie zu besserer hämodynamischer Stabilität, weniger Blutverlust und vereinfachter Transplantatimplantation beitragen.
    Notes: Summary. The experience with laterolateral cavocavostomy for hepatovenous reconstruction in liver transplantation is reviewed with and without the use of a temporary portocaval shunt. A total of 65 liver transplantations were analyzed. In 49 transplantations a laterolateral cavocaval anastomosis was performed (group I). In group II (n = 16) the same technique was used after a temporary portal caval shunt was constructed. Mean arterial pressure (mmHg): group I 128 ± 34; group II 109 ± 32. Cardiac output (l/min) decrease during the anhepatic phase was 2.3 ± 1.9 and 1.2 ± 1.5, respectively (P 〈 0.05). The peroperative blood loss measured as the number of packed cells transfused was 16.4 ± 15.8 versus 1.2 ± 2.3 (P 〈 0.04) and fresh frozen plasma 19.0 ± 14.7 versus 3.7 ± 4.0 (P 〈 0.02). Course on ICU (days), liver function tests, renal function and the need for reoperation because of bleeding were not statistically significantly different between the groups. One-year patient survival was 82.7 and 85.7 %, respectively. In conclusion, we found that despite preservation of the caval flow during hepatectomy, the additional use of a temporary portocaval shunt was advantageous with regard to peroperative hemorrhage and hemodynamic stability and can potentially facilitate implantation of the liver graft.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 370 (1987), S. 211-221 
    ISSN: 1435-2451
    Keywords: Islet transplantation ; Dogs ; Metabolic function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Pancreata von 20 Hunden wurden total excidiert, mittels einer intraductalen Kollagenaseperfusionstechnik und mechanischer Zerkleinerung zu insulinproduzierenden Pankreasfragmenten verarbeitet und als Autotransplantat in die Milz eines jeden Tieres mittels intravenösem Reflux in die V. lienalis injiziert. Bei 75% aller transplantierten Tiere wurde eine langfristige normoglykämische Stoffwechsellage hergestellt, wobei die Glucosetoleranzteste im Vergleich zu 13 Kontrolltieren pathologisch verändert waren. Der Milzinsulingehalt der erfolgreich transplantierten Tiere war auf ca. 5 bis 15% des urspünglichen Pankreasinsulingehaltes reduziert, was für die Funktionseinschränkung verantwortlich sein dürfte. Die angewandte intraductale Kollagenaseperfusionstechnik ist eine zuverlässige Methode zur Wiederherstellung einer normoglykämischen Stoffwechsellage nach totaler Pankreatektomie im Autotransplantationsmodell. Zur Steigerung der Funktionsfähigkeit des Inseltransplantates ist eine erhöhte Inselimplantationsrate erforderlich.
    Notes: Summary 20 dogs underwent total pancreatectomy. Pancreatic fragments were produced using an intraductal collagenase perfusion technique and mechanical disruption of the pancreatic gland. The resulting tissue suspension was transplanted as an autotransplant to the spleen of each animal by intravenous reflux injection into the splenic vein. In 75% of all transplanted animals long-term function with normoglycemia was restored while i.v. glucose tolerance tests were impaired as compared to 13 controls. The spleen insulin content of these sucessfully transplanted animals corresponded to only 5 to 15% of the original pancreas insulin content which was responsible for the reduced function. Intraductal collagenase perfusion is a reliable method to restore normoglycemia after total pancreatectomy in the autotransplant model using pancreatic fragments. To improve the metabolic function of the islet transplant an increased number of islets has to be implanted.
    Type of Medium: Electronic Resource
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