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  • Articles: DFG German National Licenses  (2)
  • Mitochondria  (2)
  • 1
    Electronic Resource
    Electronic Resource
    The mitochondrial transport protein superfamily (1993)
    Springer
    Journal of bioenergetics and biomembranes 25 (1993), S. 435-446 
    Details
    ISSN: 1573-6881
    Keywords: Mitochondria ; transport proteins ; sequences ; bacterial expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The ADP/ATP, phosphate, and oxoglutarate/malate carrier proteins found in the inner membranes of mitochondria, and the uncoupling protein from mitochondria in mammalian brown adipose tissue, belong to the same protein superfamily. Established members of this superfamily have polypeptide chains approximately 300 amino acids long that consist of three tandem related sequences of about 100 amino acids. The tandem repeats from the different proteins are interrelated, and probably have similar secondary structures. The common features of this superfamily are also present in nine proteins of unknown functions characterized by DNA sequencing in various species, most notably inCaenorhabditis elegans andSaccharomyces cerevisiae. The high level expression inEscherichia coli of the bovine oxoglutarate/malate carrier, and the reconstitution of active carrier from the expressed protein, offers encouragement that the identity of superfamily members of known sequence but unknown function may be uncovered by a similar route.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01108401
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Yeast Mitochondrial Carriers: Bacterial Expression, Biochemical Identification and Metabolic Significance (2000)
    Springer
    Journal of bioenergetics and biomembranes 32 (2000), S. 67-77 
    Details
    ISSN: 1573-6881
    Keywords: Mitochondria ; transport ; overexpression ; dicarboxylate carrier ; ACR1 gene ; succinate-fumarate exchange ; ARG11 gene; ornithine carrier ; arginine biosynthesis ; yeast ; metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The genome of Saccharomyces cerevisiae encodes 35 members of a family proteins thattransport metabolites and substrates across the inner membranes of mitochondria. They includethree isoforms of the ADP/ATP translocase and the phosphate and citrate carriers. At the startof our work, the functions of the remaining 30 members of the family were unknown. We areattempting to identify these 30 proteins by overexpression of the proteins in specially selectedhost strains of Escherichia coli that allow the carriers to accumulate at high levels in the formof inclusion bodies. The purified proteins are then reconstituted into proteoliposomes wheretheir transport properties are studied. Thus far, we have identified the dicarboxylate,succinate-fumarate and ornithine carriers. Bacterial overexpression and functional identification, togetherwith characterization of yeast knockout strains, has brought insight into the physiologicalsignificance of these transporters. The yeast dicarboxylate carrier sequence has been used toidentify the orthologous protein in Caenorhabditis elegans and, in turn, this latter sequencehas been used to establish the sequence of the human ortholog.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005564429242
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    Paper (German National Licenses)
    Fulltext
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