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  • 1
    Electronic Resource
    Electronic Resource
    Absence of 7-acetyl taxol binding to unassembled brain tubulin
    Amsterdam : Elsevier
    FEBS Letters 227 (1988), S. 96-98 
    Details
    ISSN: 0014-5793
    Keywords: Equilibrium dialysis ; Microtubule ; Taxol ; Tubulin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)80875-5
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of 7-acetyltaxol with different tubulin assemblies
    Amsterdam : Elsevier
    FEBS Letters 234 (1988), S. 177-180 
    Details
    ISSN: 0014-5793
    Keywords: Taxol ; Tubulin Equilibrium dialysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)81328-0
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Variation of the immunolabelling of the α1-isotubulin in the mitotic spindle ofPhysarum polycephalum (1989)
    Springer
    Protoplasma 148 (1989), S. 120-129 
    Details
    ISSN: 1615-6102
    Keywords: Tubulin ; Mitosis ; Microtubule ; Physarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immunofluorescent labelling ofPhysarum microtubules with a new antibody specific for the α1-isotubulin has been compared with the labelling with an antibody specific for β-isotubulins and an antibody with recognizes tubulin chains terminated by an aromatic amino-acid. In agreement with the known presence of only one α-isotype in amoebae and several α-isotypes in plasmodia, the immunofluorescence of the mitotic spindle was qualitatively identical, but lower in plasmodia than in amoebae. In all cases except one, there were no relative variations of immuno-fluorescence staining with the three antibodies, from metaphase to telophase, in spindles sampled. In plasmodia grown at optimal temperature, both during normal or perturbed mitosis, the immunostaining of the α1isotype decreased sharply after metaphase, while the staining obtained with the two other antibodies did not vary significantly. The immunologic determination of the relative amount of the α1-isotubulin in the tubulin pool and in isolated mitotic microtubules could not account for this observation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02079331
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  • 4
    Electronic Resource
    Electronic Resource
    The centriole cycle in the amoebae of the myxomycetePhysarum polycephalum (1986)
    Springer
    Protoplasma 132 (1986), S. 23-31 
    Details
    ISSN: 1615-6102
    Keywords: Centrioles ; Myxomycete ; Physarum ; Mitosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the amoebae of the myxomycetePhysarum polycephalum, procentrioles are formed on the anterior and posterior centrioles in early prophase. Although the relative position of the parental and procentrioles is fixed, all relative positions of the daughter and parental centrioles were observed. During the different stages of mitosis daughter centrioles elongate and acquire anterior satellites, one of the characteristic features of the anterior centrioles. All other anterior morphological characteristics appear only in telophase and early reconstruction stages. In contrast to the parental posterior centrioles, which do not change morphologically during the successive mitotic stages, the parental anterior centrioles lose their morphological characteristics in late prophase and early prometaphase and then acquire the morphological features characteristic of the posterior centrioles. Thus, the following maturation scheme is suggested: a procentriole becomes an anterior centriole during the first mitosis and a posterior centriole during the second mitosis. Since posterior features are maintained during mitosis, the posterior centriole corresponds to the final state of centriole maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275786
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  • 5
    Electronic Resource
    Electronic Resource
    Variations in the number of centrioles, the number of microtubule organizing centers 1 and the percentage of mitotic abnormalities inPhysarum polycephalum amoebae (1984)
    Springer
    Protoplasma 120 (1984), S. 20-35 
    Details
    ISSN: 1615-6102
    Keywords: Centriole ; Microtubule organizing center ; Mitosis ; Physarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several, stable amoebal strains which differ phenotypically from the diploid parental amoebal strain have been obtained in the MyxomycetePhysarum polycephalum. They were detected using their flagellation pattern as a discriminating parameter. This approach is valid since the number of flagella by phase contrast microscopy correlates with the number of anterior centrioles obtained using three-dimensional reconstructions of the nucleo-flagellar complexes from serial thin sections. The complexity of the structures of the various nucleo-flagellar complexes suggests that in these strains the duplication time of centrioles is not strictly regulated as it is in haploid amoebae. In agreement with this hypothesis, several pro-centrioles were observed in interphase amoebae. Although the anterior centrioles are linked to the mtoc 1 during interphase, the number of mtoc 1 cannot regulate the number of centrioles since some strains possess two mtoc 1 but only one pair of centrioles. Neither the number of centrioles nor the number of mtoc 1 are related to ploidy. Stable strains with one (all haploid strains), two (some diploid strains) and three (some diploid strains) mtoc 1 have been observed. Thus each mtoc 1 is duplicated once per cell cycle implying that it must possess some information which plays a role in the morphogenesis of the new mtoc 1. Except in one case, the number of mitotic abnormalities increases exponentially with the number of mtoc 1. This observation suggests that the mtoc 1 could correspond to the interphase state of the mitotic center.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287614
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  • 6
    Electronic Resource
    Electronic Resource
    Indirect immunofluorescent staining of the microtubules in interphase and mitotic amoebae of the myxomycetePhysarum polycephalum (1984)
    Springer
    Protoplasma 120 (1984), S. 43-50 
    Details
    ISSN: 1615-6102
    Keywords: Aster ; Immunofluorescence ; Mitosis ; Myxomycetes ; Physarum ; Taxol ; Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The microtubules ofPhysarum amoebae have been decorated with rat antibodies against yeast tubulin. The indirect fluorescent staining observed in interphase amoebae and in flagellated amoebae is consistent with the three-dimensional reconstructions previously deduced from electron microscopic studies. Mitotic amoebae exhibit a pattern of fluorescence which is similar to that exhibited by mammalian cells and is consistent with the previous electron microscopic studies, except that we also observe pole-pole microtubule fibers during metaphase and anaphase and the presence of a typical midbody during cytokinesis. The various types of tripolar mitosis which are observed suggest that there is a regulatory mechanism allowing the formation of pseudo-bipolar mitotic apparatuses in amoebae possessing more than two mitotic centers during mitosis. The mitotic center, located in the middle of the centrosphere, is not fluorescent after staining of the monoasters induced with taxol suggesting the absence of tubulin in the mitotic center.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287616
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  • 7
    Electronic Resource
    Electronic Resource
    Stabilization of monoasters by taxol in the amoebae ofPhysarum polycephalum (Myxomycetes) (1982)
    Springer
    Protoplasma 113 (1982), S. 44-56 
    Details
    ISSN: 1615-6102
    Keywords: Taxol ; Amoebae ; Physarum ; Myxomycetes ; Centrosphere ; Mitosis ; Microtubule ; Centriole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although the plasmodial stage of the MyxomycetePhysarum polycephalum was unaffected with 200 μM taxol, the amoebal stage was sensitive to 10 μM taxol. The first effect of taxol resulted in an accumulation of cells blocked as a monopolar centrosphere surrounded by condensed chromosomes. In 79% of cases these monoasters contained two pairs of centrioles. The mitotic block in a monopolar stage in the presence of taxol delayed the occurrence of late mitotic events such as chromosome decondensation and formation of the nuclear envelope. Escape from the monopolar centrosphere stage and formation of multinucleated amoebae involved a transient monopolar reconstruction stage in which a long microtubular bundle interacted with a small chromosomal mass outside the monoaster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283038
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  • 8
    Electronic Resource
    Electronic Resource
    Spatial relationships between centrioles and the centrosphere in monoasters induced by taxol inPhysarum polycephalum amoebae (1982)
    Springer
    Protoplasma 113 (1982), S. 69-79 
    Details
    ISSN: 1615-6102
    Keywords: Taxol ; Amoebae ; Physarum ; Myxomycetes ; Centrosphere ; Mitosis ; Centriole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Monoasters induced by taxol in the amoebae of the MyxomycetePhysarum polycephalum show an unusual tridimensional location of the centrioles. Tridimensional reconstructions of individual monoasters with either two or four centrioles show that the position of centrioles is not random. The characteristics of these monoasters suggest that the centrioles are not linked to the mitotic center in the monoaster since mitotic centers completely devoid of centrioles in adjacent or central location are observed. However, the preferential centrifugal orientation of the centrioles in the centrosphere induced by taxol suggests that centrioles are initially located in the mitotic center in agreement with the attachment of the centrioles to the mitotic center during interphase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283040
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