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  • 1
    Electronic Resource
    Electronic Resource
    Absence of 7-acetyl taxol binding to unassembled brain tubulin
    Amsterdam : Elsevier
    FEBS Letters 227 (1988), S. 96-98 
    Details
    ISSN: 0014-5793
    Keywords: Equilibrium dialysis ; Microtubule ; Taxol ; Tubulin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)80875-5
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of 7-acetyltaxol with different tubulin assemblies
    Amsterdam : Elsevier
    FEBS Letters 234 (1988), S. 177-180 
    Details
    ISSN: 0014-5793
    Keywords: Taxol ; Tubulin Equilibrium dialysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)81328-0
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  • 3
    Electronic Resource
    Electronic Resource
    The centriole cycle in the amoebae of the myxomycetePhysarum polycephalum (1986)
    Springer
    Protoplasma 132 (1986), S. 23-31 
    Details
    ISSN: 1615-6102
    Keywords: Centrioles ; Myxomycete ; Physarum ; Mitosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the amoebae of the myxomycetePhysarum polycephalum, procentrioles are formed on the anterior and posterior centrioles in early prophase. Although the relative position of the parental and procentrioles is fixed, all relative positions of the daughter and parental centrioles were observed. During the different stages of mitosis daughter centrioles elongate and acquire anterior satellites, one of the characteristic features of the anterior centrioles. All other anterior morphological characteristics appear only in telophase and early reconstruction stages. In contrast to the parental posterior centrioles, which do not change morphologically during the successive mitotic stages, the parental anterior centrioles lose their morphological characteristics in late prophase and early prometaphase and then acquire the morphological features characteristic of the posterior centrioles. Thus, the following maturation scheme is suggested: a procentriole becomes an anterior centriole during the first mitosis and a posterior centriole during the second mitosis. Since posterior features are maintained during mitosis, the posterior centriole corresponds to the final state of centriole maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275786
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  • 4
    Electronic Resource
    Electronic Resource
    Indirect immunofluorescent staining of the microtubules in interphase and mitotic amoebae of the myxomycetePhysarum polycephalum (1984)
    Springer
    Protoplasma 120 (1984), S. 43-50 
    Details
    ISSN: 1615-6102
    Keywords: Aster ; Immunofluorescence ; Mitosis ; Myxomycetes ; Physarum ; Taxol ; Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The microtubules ofPhysarum amoebae have been decorated with rat antibodies against yeast tubulin. The indirect fluorescent staining observed in interphase amoebae and in flagellated amoebae is consistent with the three-dimensional reconstructions previously deduced from electron microscopic studies. Mitotic amoebae exhibit a pattern of fluorescence which is similar to that exhibited by mammalian cells and is consistent with the previous electron microscopic studies, except that we also observe pole-pole microtubule fibers during metaphase and anaphase and the presence of a typical midbody during cytokinesis. The various types of tripolar mitosis which are observed suggest that there is a regulatory mechanism allowing the formation of pseudo-bipolar mitotic apparatuses in amoebae possessing more than two mitotic centers during mitosis. The mitotic center, located in the middle of the centrosphere, is not fluorescent after staining of the monoasters induced with taxol suggesting the absence of tubulin in the mitotic center.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287616
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  • 5
    Electronic Resource
    Electronic Resource
    The structure of the flagellar apparatus of the swarm cells ofPhysarum polycephalum (1979)
    Springer
    Protoplasma 100 (1979), S. 231-250 
    Details
    ISSN: 1615-6102
    Keywords: Cytochalasin A ; Nucleo-flagellar complex isolation ; Kinetosome ; Microtubule ; Myxomycete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Flagellation ofPhysarum polycephalum amoebae (Myxomycete) involves the formation around the two kinetosomes of a flagellar apparatus leading to a modification in the shape of the amoeba and its nucleus. A tridimensional ultrastructural model of the flagellar apparatus is proposed, based upon observation of the isolated nucleo-flagellar apparatus complex. The flagellar apparatus is composed of a non-microtubular structure (the posterior para-kinetosomal structure), five microtubular arrays and two flagella: a long anterior flagellum and a short flagellum directed backwards. The asymmetry of the flagellar apparatus is due mainly to the presence of the posterior para-kinetosomal structure on the right side of the posterior kinetosome and of the two asymmetrical microtubular arrays 3 and 4. Thus, the flagellar apparatus is right-handed. This asymmetry implies also some spatial constraints on two other microtubular arrays (2 and 5). Except in the case of the microtubular array 1 which links the proximal end of the anterior kinetosome to the nuclear membrane, the number of microtubules of each microtubular array seems to be well defined: 39, 5–6, 7–9, and 2+2 for the microtubular arrays 2, 3, 4, and 5 respectively. All the elements of the nucleo-flagellar apparatus complex are linked either directly or indirectly through bridges. Furthermore, the microtubules which composed the microtubular array 3 are linked through bridges while the microtubules of the microtubular arrays 2, 3, and 4 seem to be linked through a reticulate material. All these spatial relationships lead to a great cohesion of the nucleo-flagellar apparatus complex which appears to be a well defined structure. This suggests thatPhysarum amoebal flagellation can be a promising system to study the morphogenesis of an eucaryotic cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279314
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  • 6
    Electronic Resource
    Electronic Resource
    Stabilization of monoasters by taxol in the amoebae ofPhysarum polycephalum (Myxomycetes) (1982)
    Springer
    Protoplasma 113 (1982), S. 44-56 
    Details
    ISSN: 1615-6102
    Keywords: Taxol ; Amoebae ; Physarum ; Myxomycetes ; Centrosphere ; Mitosis ; Microtubule ; Centriole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although the plasmodial stage of the MyxomycetePhysarum polycephalum was unaffected with 200 μM taxol, the amoebal stage was sensitive to 10 μM taxol. The first effect of taxol resulted in an accumulation of cells blocked as a monopolar centrosphere surrounded by condensed chromosomes. In 79% of cases these monoasters contained two pairs of centrioles. The mitotic block in a monopolar stage in the presence of taxol delayed the occurrence of late mitotic events such as chromosome decondensation and formation of the nuclear envelope. Escape from the monopolar centrosphere stage and formation of multinucleated amoebae involved a transient monopolar reconstruction stage in which a long microtubular bundle interacted with a small chromosomal mass outside the monoaster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283038
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  • 7
    Electronic Resource
    Electronic Resource
    Spatial relationships between centrioles and the centrosphere in monoasters induced by taxol inPhysarum polycephalum amoebae (1982)
    Springer
    Protoplasma 113 (1982), S. 69-79 
    Details
    ISSN: 1615-6102
    Keywords: Taxol ; Amoebae ; Physarum ; Myxomycetes ; Centrosphere ; Mitosis ; Centriole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Monoasters induced by taxol in the amoebae of the MyxomycetePhysarum polycephalum show an unusual tridimensional location of the centrioles. Tridimensional reconstructions of individual monoasters with either two or four centrioles show that the position of centrioles is not random. The characteristics of these monoasters suggest that the centrioles are not linked to the mitotic center in the monoaster since mitotic centers completely devoid of centrioles in adjacent or central location are observed. However, the preferential centrifugal orientation of the centrioles in the centrosphere induced by taxol suggests that centrioles are initially located in the mitotic center in agreement with the attachment of the centrioles to the mitotic center during interphase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283040
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  • 8
    Electronic Resource
    Electronic Resource
    The structure of the pro-flagellar apparatus of the amoebae ofPhysarum polycephalum: Relationship to the flagellar apparatus (1980)
    Springer
    Protoplasma 103 (1980), S. 69-81 
    Details
    ISSN: 1615-6102
    Keywords: Kinetosome ; Microtubule ; Morphogenesis ; Physarum ; Myxomycete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Unflagellated amoebae ofPhysarum polycephalum (Myxomycete) possess a pro-flagellar apparatus. A tridimensional ultrastructural model of the pro-flagellar apparatus is proposed, based upon observations of thin sections of whole amoebae and of isolated nucleo-pro-flagellar apparatus complexes. The pro-flagellar apparatus is composed by the same basic elements as the fully differentiated flagellar apparatus and differs from the latter by three main aspects: the two kinetosomes of the pro-flagellar apparatus are devoid of flagella; the posterior kinetosome is directed forward, and, with the exception of microtubular arrays 1 and 5, all the other microtubular arrays (2–4) have a reduced length, although they show a normal number of constituting microtubules. We demonstrate the existence of an extension of the posterior para-kinetosomal structure which is present both in the pro-flagellar and in the flagellar apparatus. The growth of the microtubules of the pericentriolar arrays and of the flagella could be the main event which leads to the formation of the fully developed flagellar apparatus inPhysarum amoebae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276742
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