ISSN:
1432-2242
Keywords:
RAPD-PCR
;
DNA Fingerprinting
;
Sibling analysis
;
Oviposition
;
Aedes aegypti
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract There are many species in which groups of individuals encountered in the field are known to consist of mixtures of full-sibling families. We describe a statistical technique, based on the use of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers, that allows for the estimation of the number of families contained in these groups. We test the technique on full-sibling families of the mosquito Aedes aegypti, a species that distributes its eggs among several locations. Mixtures of 10 families with 15 individuals per family were analyzed using 40 RAPD-PCR loci amplified by 5 primers. Our analysis accurately estimated the number of families. The technique was accurate when the number of families was small or when family sizes were small and variable.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00211052
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