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  • Articles: DFG German National Licenses  (2)
  • Rice blast  (1)
  • Transformation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Meiotic and mitotic stability of transforming DNA in the phytopathogenic fungus Magnaporthe grisea (1992)
    Springer
    Current genetics 21 (1992), S. 55-60 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Stability ; Hygromycin B resistance ; Rice blast fungus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Magnaporthe grisea was transformed with cosmid pAN7-2 encoding hygromycin B resistance but containing no homology with the M. grisea genome. Rearrangement of the integrated DNA was detected in several hygromycin B-resistant progeny from cross Guy11-T10-1 (a single-copy integration site transformant)x2539 (sensitive wild-type parent), but not in hygromycin B-resistant progeny from four other crosses. Transformants produced typical lesions when inoculated onto host plants. Southern hybridization revealed rearrangements of integrated DNA in single conidial isolates of highcopy transformant 2539-T1-1 re-isolated from host plants, characterized by excision of one or more copies of the transforming plasmid. Plasmid loss and rearrangement were also observed within single conidial isolates derived from transformant 2539-T1-1 following ten asexual generations on non-selective agar medium. These examples of instability of integrated DNA in M. grisea transformants suggest that caution should be exercised in the use of transformation for assessing the phenotypic effects of specific introduced genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318655
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea (1997)
    Springer
    Current genetics 31 (1997), S. 361-369 
    Details
    ISSN: 1432-0983
    Keywords: Key words Repeated DNA ; Rice blast ; Fingerprinting ; Transposon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583 (Hamer et al. 1989), a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050217
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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