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  • Articles: DFG German National Licenses  (2)
  • TaqI restriction endonuclease  (1)
  • nonhistone protein  (1)
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  • Articles: DFG German National Licenses  (2)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    The effect of cycloheximide on nuclear uH2A content (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 1398-1399 
    Details
    ISSN: 1420-9071
    Keywords: Rat liver ; liver, rat ; chromatin, rat liver ; cycloheximide ; nonhistone protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of intraperitonal cycloheximide administration on acid-soluble rat liver chromatin proteins has been investigated by electrophoresis in acetic acid-urea polyacrylamide gels. A nonhistone protein, which migrates between oxidized histone H3 and histone H1 has been found tobe increased in amount following cycloheximide treatment. This protein seems to be identical with semihistone protein H24 (uH2A). A possible relationship of uH2A to the inhibition of rRNA synthesis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951910
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    High level secretion of TaqI restriction endonuclease by recombinant Escherichia coli (1999)
    Springer
    Biotechnology techniques 13 (1999), S. 803-808 
    Details
    ISSN: 1573-6784
    Keywords: TaqI restriction endonuclease ; MBP fusion protein ; secretion ; extracellular production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The production and high level secretion of TaqI restriction endonuclease using bacterial secretion signal within the malE gene was achieved by cloning the PCR-amplified gene from Thermus aquaticus into E. coli. The maltose binding protein (MBP) part of the MBP-TaqI fusion protein expressed by this construct did not interfere with the biological activity of the TaqI restriction endonuclease. E. coli XL1 carrying pH185 produced 332 U ml−1 TaqI endonuclease 81% of which was secreted into the medium without apparent cell lysis. Optimization of culture conditions and selection of the host strain were found to be important for the efficient extracellular production of this protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008938302312
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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