ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Bone remodelling is a coupled process of bone formation and resorption. This process isphysiologically controlled and involves the synthesis of bone matrix by osteoblasts and boneresorption by giant cells called osteoclasts. It includes a complex interaction of cells, with specificchemokine signalling. In order to study bone remodelling in vitro, we cultivated two precursor celllinetypes in a model coculture system on synthetic hydroxyapatite ceramic (HA) for 14 days. Themonocytes have the capacity to differentiate into osteoclast-like cells, and the bone marrow stromalcells can differentiate into osteoblast-like cells. This coculture was used to analyse the in vitro cellinteraction between monocytes and stromal cells. Furthermore, the attachment of the bone marrowstromal cells to the resorbed HA-surfaces was studied. In this in vitro study we demonstratedosteoclast-like differentiation and bone marrow stromal growth in a coculture system on a syntheticbone substitute material. Under optimal conditions, HA can be resorbed and bone marrow cells cangrow into the lacunas to form new bone. These results give important cues for the adjustment ofsynthetic bone substitute materials for optimal remodelling behaviour
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1123.pdf
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