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  • 1
    Electronic Resource
    Electronic Resource
    The disulfide bridges of the immunoreactive forms of human pancreatic stone protein isolated from pancreatic juice
    Amsterdam : Elsevier
    FEBS Letters 229 (1988), S. 171-174 
    Details
    ISSN: 0014-5793
    Keywords: (Pancreatic juice, Human) ; Disulfide bridge ; Pancreatic stone protein
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)80820-2
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  • 2
    Electronic Resource
    Electronic Resource
    N-terminal sequence extension in the glycosylated forms of human pancreatic stone protein. The 5-oxoproline N-terminal chain is O-glycosylated on the 5th amino acid residue
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 994 (1989), S. 281-284 
    Details
    ISSN: 0167-4838
    Keywords: (Human) ; Amino acid sequence ; Amino terminal 5-oxoproline ; Carbohydrate content ; O-Glycosyl-bond cleavage ; Pancreatic calculi ; Pancreatic stone protein
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-4838(89)90305-1
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  • 3
    Electronic Resource
    Electronic Resource
    Reactivation of the totally inactive pancreatic lipase RP1 by structure-predicted point mutationsThe key results were presented by C. Cambillau on September 20th, 1997 during the European meeting held at Como (Italy) on: “Lipases and lipids: structure, specificity and applications in biocatalysis.”A. Roussel and J. de Caro contributed in equal proportions to the present study. (1998)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 32 (1998), S. 523-531 
    Details
    ISSN: 0887-3585
    Keywords: pancreatic juice ; pancreatic lipase ; glycerides ; X-ray structure ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Both classical pancreatic lipase (DPL) and pancreatic lipase-related protein 1 (DPLRP1) have been found to be secreted by dog exocrine pancreas. These two proteins were purified to homogeneity from canine pancreatic juice and no significant catalytic activity was observed with dog PLRP1 on any of the substrates tested: di- and tri-glycerides, phospholipids, etc. DPLRP1 was crystallized and its structure solved by molecular replacement and refined at a resolution of 2.10 Å. Its structure is similar to that of the classical PL structures in the absence of any inhibitors or micelles. The lid domain that controls the access to the active site was found to have a closed conformation. An amino-acid substitution (Ala 178 Val) in the DPLRP1 may result in a steric clash with one of the acyl chains observed in the structures of a C11 alkyl phosphonate inhibitor, a transition state analogue, bound to the classical PL. This substitution was suspected of being responsible for the absence of DPLRP1 activity. The presence of Val and Ala residues in positions 178 and 180, respectively, are characteristic of all the known PLRP1, whereas Ala and Pro residues are always present in the same positions in all the other members of the PL gene family. Introducing the double mutation Val 178 Ala and Ala 180 Pro into the human pancreatic RP1 (HPLRP1) gene yielded a well expressed and folded enzyme in insect cells. This enzyme is kinetically active on triglycerides. Our findings on DPLRP1 and HPLRP1 are therefore likely to apply to all the RP1 lipases. Proteins 32:523-531, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Guanine nucleotide binding regulatory proteins in liver from obese humans with and without type II diabetes: Evidence for altered “cross-talk” between the insulin receptor and Gi-proteins (1994)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 54 (1994), S. 309-319 
    Details
    ISSN: 0730-2312
    Keywords: diabetes ; G proteins ; insulin ; obesity ; insulin receptors ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A novel pathway for physiological “cross-talk” between the insulin receptor and the regulatory Gi-protein has been demonstrated. We tested the hypothesis that a coupling defect between Gi and the insulin receptor is present in the liver of obese patients with and without type li diabetes. Insulin 1 × 10-9 M (∼ ED50) and 1 × 10-7 M (Max) inhibited pertussis toxin-catalyzed ADP ribosylation of Gi in human liver plasma membranes from lean and obese nondiabetic patients. However, 1 × 10-7 M insulin was without effect in membranes from patients with type II diabetes. This coupling defect was not intrinsic to Gi, since Mg2+ and GTPγS inhibited pertussis toxin-catalyzed ADP ribosylation in both diabetic and nondiabetic patients. Binding of insulin of the α-subunit and activation of the tyrosine kinase intrinsic to the β-subunit of the insulin receptor are not responsible for the coupling defect. 125I insulin binding is the same in obese patients with or without diabetes. Tyrosine kinase of the insulin receptor is decreased in diabetes. However, a monoclonal antibody to the insulin receptor (MA-20) at equimolar concentrations with insulin equally inhibits pertussis toxin-catalyzed ADP ribosylation of Gi without activating tyrosine kinase or insulin receptor autophosphorylation. Immunodetection of G-proteins suggested that Gi3α was normal in diabetes and Gi1-2α was decreased by 40% in the diabetic group as compared to the obese nondiabetic group but was normal when compared to the lean non diabetic group. We conclude that the novel pathway of insulin signaling involving the regulatory Gi proteins via biochemical mechanisms not directly involving the tyrosine kinase of the insulin receptor is altered in obese type II diabetes and offers a new target for the search of the mechanism(s) of insulin resistance.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240540307
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of the Effects of Forskolin and Dibutyryl Cyclic AMP in Neuroblastoma Cells: Evidence that Some of the Actions of Dibutyryl Cyclic AMP Are Mediated by Butyrate (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 51 (1988), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We have compared the effects of forskolin, N6,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate (dibutyryl cyclic AMP, Bt2-cAMP), and butyrate on several aspects of neuroblastoma cell physiology. The morphology of Neuro 2A cells was similar after incubation with forskolin and Bt2-cAMP, which caused extensive neurite outgrowth, whereas in the presence of butyrate some rudimentary neurites were formed but they were not nearly as extensive. All compounds produced a dose-dependent inhibition of cell proliferation, but the effect of Bt2-cAMP was more marked than that caused by forskolin, thus showing that the effect of Bt2-cAMP is due partially to the butyrate released. Acetylcholinesterase activity was lower in the cells incubated with butyrate or Bt2-cAMP than in untreated cells or in forskolin-treated cells. This suggests that cyclic AMP does not play a role in the regulation of this enzyme. Bt2-cAMP produced histone acetylation, a well-known effect of butyrate in cultured cells, whereas forskolin did not affect this modification. Consequently, the levels of thyroid hormone receptor, a nuclear protein whose concentration is regulated by butyrate through changes in acetylation of chromatin proteins, were decreased in cells incubated with Bt2-cAMP or butyrate, but were unaffected by forskolin. Butyrate elevated the concentration of histone H1°, a protein that increases in neuroblastoma cells as a result of different treatments that block cell division. The concentration of H1° in the cells treated with Bt2-cAMP was at a level intermediate between that found after treatment with butyrate and with forskolin. The present results clearly indicate that some of the effects of Bt2-cAMP on neuroblastoma cells can be attributed to the butyryl moiety of this compound rather than to the cyclic nucleotide itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1988.tb01162.x
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  • 6
    Electronic Resource
    Electronic Resource
    [.eta.5-Cyclopentadienyl]metal Tricarbonyl Pyrylium Salts: Novel Reagents for the Specific Conjugation of Proteins with Transition Organometallic Labels (1994)
    s.l. : American Chemical Society
    Bioconjugate chemistry 5 (1994), S. 655-659 
    Details
    ISSN: 1520-4812
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bc00030a022
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  • 7
    Electronic Resource
    Electronic Resource
    Three-Dimensional Vibrational Study of the Coupling between Methyl Torsion and the Molecular Frame in the S0 State of Acetaldehyde (1995)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 99 (1995), S. 8510-8515 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100021a010
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  • 8
    Electronic Resource
    Electronic Resource
    Strain-induced electron and hole lateral confinement in semiconductor heterostructures with curved heterointerfaces (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 9188-9195 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In this work we theoretically investigate the effect of the strain on the conduction and valence bands of quantum wells of two-dimensional (2D) strained semiconductor heterostructures with curved and parallel heterointerface profiles. InxGa1−xAs/GaAs heterostructures with hyperbolic heterointerface profiles are considered. The discussed structures are geometrically similar to V-shaped quantum wires, with the only difference that the well thickness is constant (absence of the crescent shape). The variation of the crystallographic orientation along the curved heterointerface profiles leads to relevant nonuniform strain fields. The nonuniform lattice deformations induce: (i) large band-gap modulations up to several tens of meV; (ii) piezoelectric effects; and (iii) lateral electron and hole potential modulations of several tens of meV. These potential modulations may allow 1D electron and hole confinement in thin InxGa1−xAs curve quantum wells (few nm thick), and should be taken into account in the calculation of the lateral confinement potentials obtained by geometrical constraints in strained V-groove-shaped quantum wires. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.362591
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  • 9
    Electronic Resource
    Electronic Resource
    Determination of the lattice strain and chemical composition of semiconductor heterostructures by high-resolution x-ray diffraction (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 4101-4110 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In order to determine the strain field of highly mismatched semiconductor heterostructures by high-resolution x-ray diffraction with high accuracy, we derived a new second-order approximation of the incidence parameter considering an arbitrary lattice deformation. Our calculations show that, almost independently from the substrate orientation and the considered material system, for low Miller-index reflections a lattice mismatch greater than 0.004 is the value for which quadratic corrections must be considered. The quadratic approximation increases the range of validity by one order of magnitude, i.e., to a lattice mismatch up to 4%. In addition, the analytical expression which relates the strain components measured by x-ray diffraction to the lattice mismatch is derived for semiconductor epitaxial layers grown on arbitrarily oriented substrate crystals. Using Vegard's rule, our formula allows us to determine the chemical composition of ternary compounds even for low-symmetry substrate orientations. We show that in this case shear strain components have a non-negligible weight in the determination of the chemical composition of ternary compounds. Several examples considering III–V, II–VI, and IV–IV semiconductor material systems are reported and discussed. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.361773
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  • 10
    Electronic Resource
    Electronic Resource
    Oocyte donation: a review (1989)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 96 (1989), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Oocyte donation provides an option for achieving pregnancy in women lacking functioning gonads, or in whom IVF techniques have failed to harvest adequate oocytes, or those who do not wish to use their own gametes because of hereditary disease. In agonadal women, artificial menstrual cycles are required before proceeding to gamete donation. A fixed cyclical steroid replacement schedule of oestradiol (E2) valerate and progesterone (P4) pessaries was initially used, but the need for synchrony between donor and recipient cycles, and the narrow window for implantation limited the transfer of fresh embryos. Donorrecipient cycle asynchrony can be overcome by using frozen-thawed embryos, or by extending the follicular phase in the recipient to widen the transfer window. Twenty-two pregnancies have now been achieved by the Monash/Epworth group, resulting in the birth of 13 healthy Infants. There were no statistically significant differences in pregnancy rates (per transfer) between transfers in natural cycles (14%, four pregnancies) and steroid replacement cycles (24%, 16 pregnancies). Five pregnancies (36%) were established in women treated with 2 mg of E2 daily 13–18 days before embryo transfer with P, starting on the day of or the day tollowing oocyte retrieval. E2 was continued for a median of 85 days (range 49–110) and P4 for a median of 86 days (range 49–133) after the supposed last menstrual period. All but one delivery was by caesarean section. There were no perinatal deaths and no ectopic pregnancies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1989.tb03342.x
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