ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Two types of expression vectors containing theP tac orP L promoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) inEscherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression ofpGH in theP tac -driven vectors, but reduced the synthesis of pGH in theP L -vectors. A leaky repression was observed in the JM103 cells containing theP tac -plasmids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01024427
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