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  • Articles: DFG German National Licenses  (11)
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  • Articles: DFG German National Licenses  (11)
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  • 1
    Electronic Resource
    Electronic Resource
    Matrix polynomial extension of the sequence-generating function method for macromolecular binding (1990)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 92 (1990), S. 5127-5135 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: A generalization of Lifson's sequence-generating function method [J. Chem. Phys. 40, 3705 (1964)] for linear chain molecules is explored. The generalization replaces the various scalar series of statistical weights, used to generate sequence partition functions, by appropriate matrix series. These matrix SGFs permit a more compact treatment of complicated macromolecular binding models than can be achieved through the standard SGF method or by using combinatorial or regular transfer matrix methods. They also lead to appreciable gains in computational speed for such models. Applications of the matrix-SGF method are presented, including selective binding of ligands to helix–coil junctions in biopolymers and sequence-selective binding of large ligands on random copolymers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.458546
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    On the determination of deoxyribonucleic acid-protein interaction parameters using the nitrocellulose filter-binding assay (1983)
    s.l. : American Chemical Society
    Biochemistry 22 (1983), S. 4730-4737 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00289a018
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The titration curve of weak polyacids (1993)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 97 (1993), S. 3623-3630 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100116a030
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Sample multiplexing for greater throughput in HPLC and related methods (1995)
    s.l. : American Chemical Society
    Analytical chemistry 67 (1995), S. 885-890 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00101a015
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    End effects of polyelectrolytes by the Mayer cluster integral approach (1982)
    s.l. : American Chemical Society
    Macromolecules 15 (1982), S. 82-86 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00229a016
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Erratum: The cell model for polyelectrolytes with added salt [J. Chem. Phys. 82, 1482 (1985)] (1985)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 83 (1985), S. 916-916 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.449878
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  • 7
    Electronic Resource
    Electronic Resource
    The cell model for polyelectrolytes with added salt (1985)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 82 (1985), S. 1482-1491 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Using a comparison theorem from the theory of elliptic equations, we obtain rigorous bounds for the exact solution to the Poisson–Boltzmann equation for the cylindrical cell model, and error bounds for approximations to the exact solution. We present a method for obtaining perturbative solutions, formally to any order, from the exact solution to the salt-free case, and we obtain an explicit solution for the potential correct to the first order. We derive formulas for thermodynamic quantities and compare them with experimental values. We find that the predicted counterion activity coefficients agree well with experimental results; however, agreement of the predicted coion activity coefficient with experiment is only qualitative. We rigorously show that as the concentration of excess salt tends to zero, and as the polyion's radius becomes very small in comparison to the cell's radius, the coion activity coefficient given by the Poisson–Boltzmann equation approaches 16/13 for polyion charge densities above a critical value; for subcritical charge densities the limiting value depends on the charge density and lies between 1 and 16/13.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.448423
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Free-sliding ligands: An alternative model of DNA-protein interactions (1981)
    New York : Wiley-Blackwell
    Biopolymers 20 (1981), S. 2225-2241 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We present an alternative to the common lattice model for nonspecific DNA-protein interactions by using ligands that translate freely along the polynucleotide instead of binding to distinct lattice sites along the polynucleotide chain. The general model we present corresponds to a one-dimensional continuum gas and is referred to as the “continuum model” to distinguish it from the general lattice model. Explicit expressions are obtained for the binding isotherm equation for two version of the continuum model, including the effects of binding-site exclusion and attractions between bound ligands. Theoretical results are compared to those obtained from the McGhee-von Hippel (1974) analysis of the lattice model with cooperative interactions between ligands occupying more than one lattice site. Practical applications of the continuum model are illustrated by analyzing (i) the noncooperative binding to single-stranded DNA by RNase (Jensen and von Hippel, 1976), and (ii) the highly cooperative binding to poly(rA) by a proteolyzed fragment of the gene 32 protein of phage T4 (Lonberg et al., 1981).
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1981.360201015
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of rodlike DNA fragments (1975)
    New York : Wiley-Blackwell
    Biopolymers 14 (1975), S. 393-408 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Native calf thymus DNA was sheared by sonication in a viscous solvent to the molecular-weight range from 3 × 104 to 3 × 105 daltons, and fractionated by gel chromatography. Number and weight average molecular weights (M̄n and M̄w) were determined for individual fractions by electron microscopy; the ratio M̄w/M̄n for the peak fraction is approximately 1.1. Sedimentation coefficients (s020,w) of these fractionated samples show an approximately linear dependence on the logarithm of the molecular weight M̄w. This behavior is that expected for rodlike molecules, and is in quantitative agreement with the theory of Yamakawa and Fujii [(1973) Macromolecules 6, 407-415] for the sedimentation coefficient of a wormlike chain with a persistence length of 625 Å, a diameter of 25 Å, and a mass per unit length of 195 daltons/Å. It appears that the wormlike coil model, without excluded volume, can represent the sedimentation behavior of DNA over the entire conformational range from rigid rod to flexible coil, using the above parameters.Equilibrium melting curves were determined for various fractions in aqueous 2.4 M tetraethylammonium bromide. A substantial broadening of the transition and decrease of the melting temperature were observed with decreasing molecular weight. Empirical expressions have been obtained relating both the transition temperature and breadth in this solvent to molecular weight.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1975.360140212
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Na+ effects on transitions of DNA and polynucleotides of variable linear charge density (1976)
    New York : Wiley-Blackwell
    Biopolymers 15 (1976), S. 893-915 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A range of linear charge densities of the ordered and disordered forms of DNA or polynucleotides can be obtained experimentally by acid or alkaline titration, or by the investigation of unusual complexes involving protonated bases or three-stranded helices. The variation of melting temperatures with Na+ concentration for various of these systems is known and in some cases is complemented by structural and thermodynamic information. We have extended the condensation-screening theory of Manning [Biopolymers, 11, 937-955 (1972)] to these systems. The stabilizing and destabilizing effects of Na+ (condensation and screening, respectively) and be independently varied, and the theory is successful in predicting the qualitative (in some cases, quanittative) behaviour that is observed. Comparison of theory and experiment indicates that the axial phosphate distance b for single-stranded polynucleotides increases with increasing pH. Values of the critical parameter ξ are obtained for the various polynucleotide structures. These values are essential for an understanding of ionic effects on charged ligand-polynucleotide interactions.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1976.360150507
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    Paper (German National Licenses)
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