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EFFECT OF DEPLETION OF SERUM CALCIUM BY EGTA ON CANINE PANCREATIC SECRETION INDUCED BY DOPAMINE AND BY SECRETIN (1974)

Iwatsuki, K. ; Furuta, Y. ; Hashimoto, K.

Oxford, UK : Blackwell Publishing Ltd

Clinical and experimental pharmacology and physiology 1 (1974), S. 0

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ISSN: 1440-1681

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: SUMMARY 1. Pancreatic secretion has been monitored in the isolated, blood-perfused canine pancreas, and the effects of depletion of serum calcium by infusion of EGTA on the increases in secretion produced by intra-arterial injections of dopamine and secretin have been investigated.2. Under resting conditions in preparations in dogs fasted for 24 h, the mean rate of pancreatic secretion was 16.4 μ1/min (s. e. m. = 2, n= 12). The mean concentrations of protein, bicarbonate and chloride in the pancreatic juice were 53.8 mg/ml (s. e. m. = 4.5), 18.0 mmol/1 (s. e. m. = 1.1) and 122.5 mmol/1 (s. e. m. = 7.5), respectively. Infusion of EGTA had no effect on resting secretion.3. Secretion elicited by dopamine or secretin was diminished about 50% during the intra-arterial infusion of EGTA (10−2 mM/ml) in the perfusing blood. The protein concentration in the secretion was diminished to a similar extent. The concentrations of bicarbonate and chloride in the pancreatic juice was not modified by EGTA infusion.4. Concomitant infusion of an equimolar concentration of CaCl2 solution abolished the inhibitory effects of EGTA infusion on the secretory responses to dopamine or secretin.5. The results suggest that dopamine and secretin influence the exocrine secretions of the pancreas by actions on both acinar and ductular cells. Acinar cell secretion is more susceptible to depletion of serum calcium levels than is secretion from ductular cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1440-1681.1974.tb00551.x

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Pulse response in an immobilized-enzyme column: Theoretical method for predicting elution curves (1980)

Adachi, S. ; Hashimoto, K. ; Matsuno, R. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 22 (1980), S. 779-797

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A theoretical method for predicting the elution profile of a pulse response in an immobilized-enzyme column is proposed. The method is based on a mass balance model, which is extensively used in gel chromatography. To test the method, a pulse of sucrose solution was applied to a column of spherical acrylamide gel in which was entrapped invertase from yeast, and it was eluted with 0.05M acetate buffer at pH 5.0. The elution curves of the substrate and the product were in fairly good agreement with the theoretically calculated ones. The method was extended to the system of reversible as well consecutive reactions.

Additional Material: 9 Ill.