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  • Electronic Resource  (6)
  • 1985-1989  (6)
  • 1988  (6)
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  • Electronic Resource  (6)
Years
  • 1985-1989  (6)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Intraspecific gametosomatic hybridisation in Petunia hybrida (1988)
    Springer
    Plant cell reports 7 (1988), S. 17-18 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following PEG and high pH induced fusion of haploid tetrad protoplasts of a normal purple flowered variety of P. hybrida with cell suspension protoplasts of a nuclear albino mutant of the variety Blue Lace, triploid gametosomatic hybrid plants were recovered. These hybrids possessed an intermediate floral morphology and the expected chromosome number of 2n=3x=21. Selection was based on the fact that pollen tetrad protoplasts failed to divide in culture and that, following complementation to chlorophyll proficiency in the gametosomatic hybrid, the hybrid cells were visualised against a background of albino cells of the variety Blue Lace. The production of such gametosomatic hybrid plants in Petunia has shown that the concept of gametosomatic hybridisation can be extended to genera other than Nicotiana and that alternative selection strategies are available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272968
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Intra- and interspecific gametosomatic hybridisation within the genus Petunia (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 197-200 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following PEG and high pH induced fusion, intraspecific gametosomatic hybrid plants (pollen tetrad protoplasts of a normal purple flowered variety of P. hybrida fused with cell suspension protoplasts of a nuclear albino mutant of the variety Blue Lace) and interspecific gametosomatic hybrid plants (tetrad protoplasts (as above) fused with cell suspension protoplasts of a nuclear albino mutant of P. parviflora) were recovered. Hybrid plants of both combinations possessed an intermediate vegetative and floral morphology with chromosome numbers of 2n=3x=21 and 2n=3x=25 respectively. Hybrid cells were in both systems identified as green colonies against an albino background as a result of complementation to chlorophyll proficiency. Pollen tetrad protoplasts did not divide. The production of such plants at the intra- and interspecific level in Petunia has shown that the concept of gametosomatic hybridisation can be extended to genera other than Nicotiana. An alternative selection strategy is available to that as used earlier for Nicotiana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040086
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Isomeric yield ratio of146La and84Br in the thermal neutron induced fission of235U (1988)
    Springer
    Journal of radioanalytical and nuclear chemistry 126 (1988), S. 479-479 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02164551
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the transposon carrying the STII gene of enterotoxigenic Escherichia coli (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 490-495 
    Details
    ISSN: 1617-4623
    Keywords: Tn4521 ; Escherichia coli enterotoxin STII gene ; IS2 ; DNA transposition ; Replicon fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Escherichia coli enterotoxin STII gene is carried by Tn4521. The terminal repeats of Tn4521 are composed of IS2 sequences; however, neither repeat is a complete IS2. In order to determine how this seemingly defective transposon could transpose, mutations were generated within Tn4521 to determine the regions essential for transposition. The left terminal repeat region was found to be non-essential, but the right terminal repeat area was demonstrated to be crucial for transposition. Within the right terminal repeat area is an open reading frame (ORF), capable of encoding a 159 amino acid protein, which was shown by frameshift mutation analysis to be required for transposition. This protein may be the transposase of Tn4521. A pair of 11 bp repeat sequences flanking the ORF was also found to be important. The right 11 bp repeat is part of the left IS2 terminal sequence, and the left 11 bp repeat is located about 300 bp upstream from the right IS2 terminal sequence located within the right terminal repeat region. The results of this study suggest that Tn4521 is a functional transposon and that the sequence including this pair of 11 bp sequences plus the intervening sequence is a transposable element which may be responsible for Tn4521 transposition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330485
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Stationary phases (1988)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 330 (1988), S. 683-689 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Several oligomeric or polymeric 1,8-disilyloctane phases, prepared by the hydrosilation of 1,7-octadiene with dichloromethylsilane or chlorodimethylsilane followed by hydrolysis and covalent-bonded onto 10 μm silica, were examined. Chromatographic properties for these phases and a RP-18 phase were compared by use of several aromatics and aliphatics. The results indicated that the effect of polymeric phases on retention and selectivity in reversed-phase liquid chromatography was more apparent than that of RP-18. In addition, a representative oligomeric 1,8-disilyloctane phase with the safrole ligand as terminal group was also tested and evaluated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00467689
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Stationäre Phasen (1988)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 330 (1988), S. 690-693 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A method for the preparation of squalene ligand chemically bonded to silica gel is described. The chromatographic properties of the material for column liquid chromatography were examined by use of polyaromatics and alkylbenzenes. Owing to the existence of a Π — Π-interaction between squalene ligand and solute, the retention mechanism and the elution order for some solutes on this column was found to differ from those obtained on a RP-18 column.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00467690
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    Paper (German National Licenses)
    Fulltext
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