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  • Electronic Resource  (3)
  • 1995-1999  (3)
  • 1985-1989
  • 1995  (3)
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  • Electronic Resource  (3)
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  • 1995-1999  (3)
  • 1985-1989
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  • 1
    Electronic Resource
    Electronic Resource
    Localization of ubiquitin in anthers and pistils of Nicotiana (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 123-128 
    Details
    ISSN: 1432-2145
    Keywords: Anther ; Pistil ; Plant reproduction ; Pollen Transmitting tissue ; Ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ubiquitin-conjugated compounds were localized in anthers and pistils of Nicotiana alata by immuno-cytochemistry. In young anthers, antibodies to ubiquitin bound to callose cell walls surrounding pollen mother cells and to organelles in the endothecium. At the freespore stage, antibodies bound to circular-cell cluster cells subtending the stomium and to organelles and cell walls of endothecial cells. Near anther dehiscence, locular material was labeled. In pistils, cell walls of stylar transmitting tissue were labeled in a beaded pattern. Antibodies bound to a thin layer surrounding ovules, to the lining of embryo sacs, to cytoplasm of eggs and synergids, and to starch grains in central cells. Sites of localization were tissue- and time-specific, suggesting a regulatory role for ubiquitin in development of reproductive structures in flowering plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00242254
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Striped projections of the outer membrane of the generative cell in Convallaria majalis pollen (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 223-227 
    Details
    ISSN: 1432-2145
    Keywords: Pollen ; Generative cell ; Cytoskeleton ; Motility ; Myosin immunogold labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In pollen grains of Convallaria majalis the outer membrane of the generative cell (GC) is the inner membrane of the vegetative cell (VC). Striped projections (SP) at the cytoplasmic face of the outer membrane of the GC were revealed by chemical fixation and also by a rapid freeze-fixation and freeze-substitution. The projections, located in groups on the protruding lobes of the GC, were arranged parallel to each other and were equally spaced (40 nm apart). The length of the SP, estimated from grazing sections of GC, was 400 nm. Each projection was composed of T-shaped elements, about 35 nm high, spaced at an average distance of 25 nm. SP were observed in mature, hydrated, activated and germinated pollen grains and seemed to be associated with microtubules and microfilaments of the VC. No evidence exists yet of SP on the sperm cell membrane. Immunogold labelling with anti-myosin antibodies showed many gold particles attached preferentially to the surface of the protruding lobes of the GC in the area of the projections. These results may suggest that the SP of Convallaria GC contain myosin-like protein and play an important role in the motility of the GC during pollen tube growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228941
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural immunolocalization of periodic pectin depositions in the cell wall ofNicotiana tabacum pollen tubes (1995)
    Springer
    Protoplasma 187 (1995), S. 168-171 
    Details
    ISSN: 1615-6102
    Keywords: Cell wall ; Immunocytochemical localization ; JIM5 antibody ; Pectins ; Pollen tube
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The monoclonal antibody (MAb) JIM5, marking acidic pectins, was used to localize ultrastructurally pectin molecules in the pollen tube wall ofNicotiana tabacum. Longitudinal sections of LR-White embedded pollen tubes were exposed to antibody treatment; accumulations of pectins were identified by counting the density of the gold particles representing the pectin epitopes along the pollen tube wall. Significant accumulations of gold grains were marked and the distances between them were measured. In many pollen tubes a more or less regular distribution of the accumulations was observed along the tube indicating a periodical deposition of pectin. The distances between the accumulations were 4–6 μm. Most of the label was found in the inner part of the outer layer of the bilayered cell wall. These findings correspond to and confirm the earlier observation by our group reporting ring-shaped periodical deposits in pollen tubes after immunofluorescence labelling with the MAb JIM5 under the confocal laser scanning microscope.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280245
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    Paper (German National Licenses)
    Fulltext
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