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  • Electronic Resource  (5)
  • 2000-2004
  • 1995-1999  (5)
  • 1997  (5)
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  • Electronic Resource  (5)
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  • 2000-2004
  • 1995-1999  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Frequency doubling in two-component self-assembled chromophoric waveguide structures (1997)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 70 (1997), S. 1941-1943 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Self-assembled chromophoric multilayers having large second-order optical nonlinearities can be combined with linear guiding materials such as polymethylmethacrylate to produce two-component frequency doubling waveguides. This approach introduces considerable flexibility in optimizing the trade-off between the overlap of waveguide mode profiles and linear absorption. Extremely low waveguide propagation losses can be achieved because electric field poling and the accompanying poling-induced optical scattering are obviated. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.118786
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and expression of a Penicillium citrinum allergen with sequence homology and antigenic crossreactivity to a hsp 70 human heat shock protein (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Penicillium citrinum has been identified as the most prevalent airborne Penicillium species in the Taipei area. However, detailed studies on allergens of this ubiquitous Penicillium species are still lacking.Objective For the characterization of allergens of this prevalent Penicillium species, molecular cloning and expression of the allergen genes of P. citrinum were performed in the present study.Methods Molecular cloning of the allergen genes was performed by using a λUni-Zap XR cDNA library of P. citrinum and serum from an asthmatic patient. The cloned cDNA was excised from the phage vector as a recombinant pBluescript phagemid and sequenced. The cDNA of the IgE-binding clone was expressed as fusion protein with the gultathione-S-transferase. The corresponding natural allergen was analysed by absorption immunoblotting using monoclonal antibody and serum from asthmatic patient. The frequency of IgE-binding to the allergen cloned was analysed by dot immunoassay using recombinant allergen and by immunoblotting using the whole extract of P. citrinum.Results In the screening of cDNA library of P. citrinum using serum from an asthmatic patient, IgE-binding cDNA clones designated SC4 and XL were obtained. The 5′-truncated, 0.7-kb and 1.7-kb cDNA inserts of clones SC4 and XL contained open reading frames of 163 and 503 amino acids, respectively. On alignment, the deduced amino acid sequences showed that 97 (60%) of the 163 amino acids and 376 (75%) of the 503 amino acids were identical to the corresponding amino acid sequence of the human heat shock protein in the hsp70 family. Both recombinant SC4 and XL showed positive SDS-PAGE-immunoblot reactivity to a monoclonal antibody MA3-006 against the human hsp 70 protein. For characterization of the corresponding natural allergen, immunoblotting reactivities of MA3–006 and IgE antibodies to the 70kDa component of P. citrinum have been shown to be disappeared after absorption of these antibodies with the recombinant SC4 protein. Sera from 14 (41%) of 34 Penicillium-allergic patients showed IgE-binding to the recombinant XL protein and the 70kDa component in the extract of P. citrinum.Conclusion Results obtained suggest that hsp 70 is an allergen of P. citrinum and that clones SC4 and XL contain partial cDNAs of this allergen gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1997.tb01197.x
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the 33-kilodalton major allergen of Penicillium citrinum by using MoAbs and N-terminal amino acid sequencing (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The 33 kD component has been identified as a major allergen ofPenicillium citrinum, the most prevalent Penicillium species in the Taipei area of Taiwan.Objective This study analyses the isoforms, antigenic cross-reactivity and the N-terminal amino acid sequence of the 33 kD allergen of P. citrinum.Methods The composition of isoforms and antigenic cross-reactivity was analysed by SDS-PAGE and 2D-immunobIotting using MoAbs generated. The N-terminal sequence was analysed by using an automatic gas/liquid phase sequencer.Results Two MoAbs (55A and 34H) against the 33 kD allergen were generated in the present study. In addition to the 33 kD component. MoAb 34H also showed immunoblot reactivity to other components in the crude extract of P. citrinum. Analysed by 2D-immunoblotting. at least six different isoforms of the 33 kD component with pl values ranging from 6.75 to greater than 7.0 were shown to be reactive to both MoAbs and IgE antibodies in serum of an asthmatic patient. Different immunoblot patterns were observed when both MoAbs were reacted with four different strains of P. citrinum used in the present study. Among another six different Penicillium and four different Aspergillus species tested, only an immunoblot reactivity of MoAb 55 A to the 33 kD component of P. brevicompactum was observed. In 2D-immunoblotting. components of P. brevicompactum with an MW of about 33 kD and pi values similar to those of the 33 kD component of P. citrinum reacted with MoAb 55A and IgE antibodies in serum of the asthmatic patient. The N-terminal amino acid sequence of the 33 kD component of P. citrinum was determined to be ANVVQSNVP which was identical to the first 9 N-terminal amino acids of a heat-labile alkaline serine proteinase from P. citrinum.Conclusion Results obtained in the present study suggest that the 33 kD major allergen of P. citrinum may be an alkaline serine proteinase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1997.tb00676.x
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the allergen Der f 7 from house dust mite extracts by species-specific and crossreactive monoclonal antibodies (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The group 7 mite allergens react with IgE in 50% of sera from allergic patients.Objective To determine the molecular and antigenic characteristics and heterogeneity of Der f 7 in mite extracts.Methods Monoclonal antibodies (MoAbs) produced from mice immunized with recombinant Der f 7 were examined for crossreactivity to Der p 7 and then used for immunoblotting of 1 and 2-D gel electrophoresis. Deglycosylation was studied with N-glycosidase-F and N-terminal sequencing by Edman degradation. The epitopes of the monoclonal antibodies were compared by cross-inhibitory immunoassays.Results Immunoblotting of D. farinae extracts with all the anti Der f 7 MoAbs showed major reactivities at 31, 30 and 25 kDa. The strongest immunostaining was at 25 kDa which contrasted with Der p 7 where the 31 and 30 kDa bands were strongest. The relative strength of staining however varied between extracts. The 31 and 30 kDa components were glycosylation products of the 25 kDa form which had the N-terminal sequence predicted from cDNA analysis. Two MoAbs stained an 18 kDa band consistent with a degradation product. The 2-D gels showed that different components with pls from 5.6–6.4. Both species-specific and Der p 7 crossreactive MoAbs were produced and a two-site ELISA assay for detecting group 7 allergen was developed with MoAbs recognizing different epitopes.Conclusions Der f 7 has been defined by its natural N-terminal sequence and MoAbs. It apparently exists as different glycosylation and degradation products in mite extracts, the relative abundance of which differs with different preparations. A two-site ELISA to measure the allergen was developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.1997.650890.x
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  • 5
    Electronic Resource
    Electronic Resource
    Tool-path generation for pockets with freeform curves using Bezier convex hulls (1997)
    Springer
    The international journal of advanced manufacturing technology 13 (1997), S. 109-115 
    Details
    ISSN: 1433-3015
    Keywords: Convex hull property ; Freeform curve ; NC machining ; Overcut ; Pocket
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The objective of this paper is to generate tool paths for pockets with freeform curves, i.e. Bezier curves or B-spline curves. Time efficiency and overcut avoidance are two of the most important factors in NC machining. A method, based on the convex hull property of Bezier curves, is thus developed so that the portion of the boundary defined by a Bezier curve is replaced by parts of its convex hull on the cavity side. Therefore, the new pocket boundary, redefined by only segments of straight lines, can be solved efficiently based on current algorithms without overcuts. Cutting tool paths can be generated for pockets with islands avoiding computation of higher degree curve/curve intersections. Furthermore, recursive subdivisions on Bezier curves are used to improve the accuracy of the cut with an allowance criterion based on sizes of convex hulls. The portion of the boundary defined by a B-Spline curve is transformed into piecewise Bezier curves. The tool-path generation for pockets with B-spline curves can thus be solved by reducing the problem to one of pockets with Bezier curves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01225757
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