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  • Electronic Resource  (2)
  • 1995-1999  (2)
  • 1955-1959
  • 1998  (2)
  • PACS: 61.48.+c; 68.35.-p; 68.35.Bs  (1)
  • Polymerase chain  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Perivascular T cells are infected with HTLV-I in the spinal cord lesions with HTLV-I-associated myelopathy/tropical spastic paraparesis: double staining of immunohistochemistry and polymerase chain reaction in situ hybridization (1998)
    Springer
    Acta neuropathologica 96 (1998), S. 340-346 
    Details
    ISSN: 1432-0533
    Keywords: Key words Immunohistochemistry ; Polymerase chain ; reaction in situ hybridization ; HTLV-I-associated ; myelopathy/tropical spastic paraparesis ; Double ; staining ; Fresh frozen sections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract HTLV-I-infected cells play an important role in pathogenesis HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Our previous studies of quantitative polymerase chain reaction (PCR) and in situ PCR suggested that T cells infiltrating in the spinal cord lesion were infected with HTLV-I. To elucidate the localization of HTLV-I proviral DNA directly, we performed double staining using immunohistochemistry and PCR in situ hybridization (PCR-ISH). Fresh frozen sections of the spinal cord from four HAM patients taken at autopsy were first immunostained with antibodies to pan T cells (UCHL-1), macrophages (KP-1) and helper/inducer T cells (OPD4). Then PCR-ISH was carried out with specific primers and probe for the HTLV-I pX region. UCHL-1-positive cells were noted around perivascular areas and, to some extent, in the parenchyma. Of the UCHL-1-positive cells, 9.4% (case 1), 9.6% (case 2), 1.1% (case 3) and 6.7% (case 4) became positive in HTLV-I PCR-ISH. UCHL-1-negative cells were HTLV-I PCR-ISH negative and almost all KP-1-positive cells were HTLV-I negative. HTLV-I was localized to OPD4-positive cells in examined lesions of cases 2 and 4. These data are a direct demonstration of HTLV-I proviral DNA localizing to infiltrated T cells in HAM/ TSP spinal cord lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050903
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  • 2
    Electronic Resource
    Electronic Resource
    AFM observation of surface reconstruction of C60 single crystals (1998)
    Springer
    Applied physics 66 (1998), S. S763 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 61.48.+c; 68.35.-p; 68.35.Bs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: 60 single crystals. The (111) and the (001) faces of C60 single crystals were illuminated with photons with energies below the band gap (generation of Frenkel-type excitons) or above the band gap. In the case of sub-band-gap illumination, reconstructed surfaces formed on the crystals. The reconstruction was represented by fringes running in the [11 ] and [0 0] directions on the (111) and (001) surfaces, respectively. As a result of supra-band-gap illumination, however, there appeared only cracks, without any fringes, which were oriented in directions characteristic of each face. The results suggest that the photo-induced surface reconstruction of C60 single crystals is induced via the recombination of Frenkel-type excitons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390051238
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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