ISSN:
1432-2013
Keywords:
Flow motion Indo-1/AM Intracellular calcium Intravital microscopy Ischemia/reperfusion Local flow pattern
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Accumulation of intracellular free calcium (Ca2+ i) may play an essential role in the ischemia/reperfusion injury of skeletal muscle. Although it has been shown that Ca2+ i levels significantly increase during ischemia/reperfusion, it is still a matter of debate whether Ca2+ i increases during ischemia alone. It was the aim of this study to monitor the in vivo Ca2+ i levels in the rat spinotrapezius muscle during ischemia of varying duration and reperfusion, using a ratiometric fluorescence technique, and to investigate the relationship between the postischemic flow patterns and Ca2+ i, if any. The muscle was loaded with Indo-1/AM and imaged by a cooled digital camera. Pre- and postischemic tissue perfusion was assessed by means of an analogue camera. Our results show that short-term ischemia (5, 15 and 30 min) and subsequent reperfusion (60 min) does not alter Ca2+ i homeostasis and that tissue perfusion promptly recovers after the insult. One or two hours of ischemia resulted in changes in Ca2+ i levels, varying from preparation to preparation; increases in some and no changes in others. In these preparations three distinct flow patterns – normal, compromised and no-reflow – could be distinguished during the 60-min reperfusion. Our main conclusion is that in skeletal muscle Ca2+ i levels may increase, the increase probably depending on the muscle fiber type exposed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240000287
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