ISSN:
1432-203X
Schlagwort(e):
Key words Auxin shock
;
2
;
4-D
;
Daucus carota
;
Somatic embryogenesis
;
Stress responses
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract This study describes the formation of somatic embryos directly on the surface of hypocotyl sections of Daucus carota L. after exposure to 450 μM of 2,4-D for 2 h, followed by culturing without 2,4-D for 2 weeks. A search for differentially expressed genes immediately after the 2,4-D treatment resulted in the identification of two genes, Dchsp-1 and Dcarg-1. Dchsp-1 has homology to low-molecular-weight heat-shock proteins, while Dcarg-1 has homology to auxin-regulated genes. Dchsp-1 was expressed upon the exposure to 2,4-D treatment but also responded to general stress responses. Dcarg-1 responded primarily to auxin; however, a parallel relationship between the expression of Dcarg-1 and the formation of somatic embryos on the hypocotyl sections was observed. It seems the exposure to auxin shock could serve as a trigger in inducing cell division in the epidermal cells and promoting their further differentiation to somatic embryos. These results are discussed in relationship to our current understanding of the molecular basis of somatic embryogenesis.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002990050772
Permalink