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  • Electronic Resource  (2)
  • 2000-2004  (2)
  • Key words:POU, Sox, Pax, ganglia, mollusk, lophotrochozoan.  (1)
  • Key words:Penaeus monodon, shrimp, chitinase, gene expression  (1)
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  • Electronic Resource  (2)
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  • 2000-2004  (2)
Year
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  • 1
    Electronic Resource
    Electronic Resource
    The Penaeus monodon Chitinase 1 Gene Is Differentially Expressed in the Hepatopancreas During the Molt Cycle (2000)
    Springer
    Marine biotechnology 2 (2000), S. 126-135 
    Details
    ISSN: 1436-2236
    Keywords: Key words:Penaeus monodon, shrimp, chitinase, gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: We have isolated a full-length chitinase complementary DNA from the tiger shrimp Penaeus monodon that encodes a 621 amino acid protein possessing the functional domains of the chitinase protein family. The Penaeus monodon chitinase 1 (PmChi-1) gene product is 81.8% identical to a chitinase 1 protein expressed in the hepatopancreas of Penaeus japonicus. Analysis by reverse transcription–polymerase chain reaction (RT-PCR) indicates that PmChi-1 messenger RNA is detectable in the hepatopancreas and the gut. PmChi-1 expression during the molt cycle fluctuates markedly, with lowest mRNA levels at stages A1, C, and D3; there is a dramatic increase in transcript abundance at the D2 stage. Using the same tissues and molt stages, RT-PCR analyses of genes encoding other digestive enzymes (trypsin, chymotrypsin, and cathepsin L), a muscle structural protein (tropomyosin II), and housekeeping proteins (elongation factor II and GTP-binding protein) indicate that PmChi-1 is expressed in a distinct tissue-specific and stage-specific manner. The other digestive enzyme genes are expressed in a similar spatiotemporal pattern, but none exhibited a dramatic increase in transcript abundance at stage D2. Increased expression of PmChi-1 at D2 suggests that hepatopancreas-expressed chitinase is involved in the degradation of endogenous chitin in the gut peritrophic membrane prior to molting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101269900016
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of POU, Sox, and Pax Genes in the Brain Ganglia of the Tropical Abalone Haliotis asinina (2000)
    Springer
    Marine biotechnology 2 (2000), S. 545-557 
    Details
    ISSN: 1436-2236
    Keywords: Key words:POU, Sox, Pax, ganglia, mollusk, lophotrochozoan.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: In gastropod mollusks, neuroendocrine cells in the anterior ganglia have been shown to regulate growth and reproduction. As a first step toward understanding the molecular mechanisms underlying the regulation of these physiological processes in the tropical abalone Haliotis asinina, we have identified sets of POU, Sox, and Pax transcription factor genes that are expressed in these ganglia. Using highly degenerate oligonucleotide primers designed to anneal to conserved codons in each of these gene families, we have amplified by reverse transcriptase polymerase chain reaction 2 POU genes (HasPOU-III and HasPOU-IV), 2 Sox genes (HasSox-B and HasSox-C), and two Pax genes (HasPax-258 and HasPax-6). Analyses with gene-specific primers indicated that the 6 genes are expressed in the cerebral and pleuropedal ganglia of both reproductively active and spent adults, in a number of sensory structures, and in a subset of other adult tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101260000039
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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