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  • Electronic Resource  (3)
  • 2000-2004  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Processing of POMC peptides by dermal microvascular endo-thelial cells: implication for EC biologic functions and skin inflammation (2004)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Experimental dermatology 13 (2004), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Dermal microvascular endothelial cells (ECs) are both source and target of the pro-opiomelanocortin (POMC) peptides ACTH and α-melanocyte-stimulating hormone (α-MSH). The availability of neuropeptides as important modulators of innate and adaptive immune responses is controlled by neuropeptide-specific peptidases such as neutral endopeptidase (NEP) or angiotensin-converting enzyme (ACE). In this study, we have tested the possibility that NEP or ACE expressed by ECs may influence the local bioavailability of POMC peptides. Incubation of ACTH1−39 with cell membranes prepared from the high NEP-/low ACE-expressing microvascular EC line 1 (HMEC-1) or from low NEP-/high ACE-expressing primary human dermal ECs (HDMECs) for 30–480 min resulted in a decrease in ACTH immunoreactivity (IR) over time in membrane supernatants that could be partially blocked with NEP inhibitors as detected by radioimmunoassay. In parallel, α-MSH IR increased peaking after 60 min. Fragments generated by incubation of HMEC-1 or HDMEC membranes with ACTH1−39, ACHT1−24 or α-MSH for 1–120 min were further analysed by mass spectroscopy. HMEC-1 membranes generated peptide products which could be altered by inhibition of NEP, but not ACE. Likewise, HDMEC membranes fragmented ACTH similar to HMEC-1 membranes in the presence of NEP inhibitors. Some of the proteins can be assigned to regular proteolytic cleavage, while others seem to be modified. Importantly, HMEC-1 and HDMEC membranes also slowly degraded α-MSH, suggesting that EC proteolytic peptidases locally control ACTH/α-MSH bioavailability, which may be important in controlling cutaneous inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0906-6705.2004.0212cc.x
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  • 2
    Electronic Resource
    Electronic Resource
    Die Leberregeneration (2000)
    Springer
    Der Onkologe 6 (2000), S. 348-355 
    Details
    ISSN: 1433-0415
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Die Leber, die größte Drüse des menschlichen Organismus, besteht aus Hepatozyten und einer Reihe von Nicht-Parenchymzellen. Die Leberzelle als Träger der wesentlichen metabolischen Funktionen, ist eine hochdifferenzierte Zelle, die sich nur 1–2mal in der Lebensspanne eines Tieres teilt. Um so erstaunlicher ist die außerordentliche Fähigkeit der Leber, sich nach traumatischer, chemischer oder toxischer Schädigung zu regenerieren. Diese Erneuerung von Lebergewebe kann einerseits auf einer Zellhyperplasie, d. h. einer Zunahme des Volumens durch Zunahme der Zellzahl oder auf einer Proliferation und Differenzierung von Lebervorläuferzellen beruhen. Die Regeneration der Leber unterliegt sowohl positiven, wie auch negativen Regulationsmechanismen. An dem komplexen Vorgang der Leberregeneration beteiligen sich nicht nur Hepatozyten, sondern auch Nicht-Parenchymzellen und mit ihnen verschiedene Wachstumsfaktoren, die vor allen Dingen im portalvenösen Blut nachzuweisen sind. Die vorliegende Arbeit beinhaltet sowohl klinische als auch pathophysiologische Aspekte und gibt einen Überblick über den heutigen Wissensstand der Leberregeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007610050515
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  • 3
    Electronic Resource
    Electronic Resource
    Lamellar-to-nematic phase transition in a lipid-surfactant mixture (2000)
    Springer
    The European physical journal 3 (2000), S. 377-388 
    Details
    ISSN: 1292-895X
    Keywords: PACS. 61.30.Jf defects in liquid crystals - 64.70.Md Transitions in liquid crystals - 87.16.Dg Membranes, bilayers, and vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: A lyotropic system, consisting of a lecithin (DMPC) and a non-ionic surfactant (C12E5) in water was studied. The system exhibits a lamellar-to-nematic phase transition. The nematic phase appears as the temperature is decreased and only exists in a very limited temperature and concentration range, for specific lipid-to-surfactant ratios. While a lamellar phase is found at higher temperatures in both mixed and pure C12E5 systems, the transition to the nematic phase at lower temperatures coincides with a micellar phase in the pure C12E5 system. The transition appears to be driven by the strong temperature dependence of the surfactant film spontaneous curvature. The structural properties of the lamellar phase close to the lamellar-to-nematic boundary have been studied by polarised light microscopy and small-angle neutron and X-ray scattering experiments. The signature of a helical defect with Burgers vector of magnitude 2 is apparent in our data, close to the lamellar-to-nematic phase transition. The proliferation of screw dislocations in the lamellar phase might be a plausible mechanism for driving this transition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101890070008
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    Paper (German National Licenses)
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