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  • Electronic Resource  (6)
  • 1995-1999  (1)
  • 1990-1994  (5)
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  • Electronic Resource  (6)
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  • 1995-1999  (1)
  • 1990-1994  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Crystallization of the RNA guanylyltransferase of Chlorella virus PBCV-1 (1997)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 53 (1997), S. 482-484 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: RNA guanylyltransferase, or capping enzyme (E.C. 2.7.7.50) catalyzes the transfer of GMP from GTP to diphosphate-terminated RNA to form the cap structure GpppN. Chlorella virus capping enzyme expressed in E. coli has been purified, treated with GTP and crystallized. X-ray diffraction data have been collected from these crystals as well as for a mercury derivative obtained by soaking the crystals in thimerosal. Selenomethionine RNA guanylyltransferase was purified and crystallized in a similar fashion. The space group is C2221 and the cell parameters are a = 93.3, b = 214.9, c = 105.8 Å. Two Hg atoms and two subsets of Se atoms have been localized using difference Patterson and Fourier methods, suggesting that there are two molecules per asymmetric unit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S090744499700231X
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    X-ray analysis of metal-substituted human carbonic anhydrase II derivatives (1994)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 93-100 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Metal-substituted crystals of human carbonic anhydrase II belonging to space group P21 with cell dimensions a = 42.7, b = 41.7, c = 73.0 Å and β = 104.6° were analyzed crystallographically. The resolution limit ranged from 1.82 to 1.92 Å with high completeness (86.2–90.7%). Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination around the metal both at pH 6 and pH 7.8, similar to the native zinc enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and manganese(II) derivatives showed increased coordination number around the metal ion. Whereas the copper is best described as penta-coordinated, the nickel and manganese are best described as hexa-coordinated. The results are briefly compared with spectroscopic observations and our current view on carbonic anhydrase catalysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444993008790
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Wild-type and E106Q mutant carbonic anhydrase complexed with acetate (1994)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 101-104 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The molecular structures of the acetate complexes of wild-type human carbonic anhydrase II (HCAII) and of E106Q mutant human carbonic anhydrase II were solved with high completeness (89–91%) to 2.1 and 1.9 Å resolution, respectively. Both wild-type and mutant enzyme crystallize in space group P21 with cell dimensions a = 42.7, b = 41.7, c = 73.0 Å and β = 104.6°. The altered active-site hydrogen-bond network caused by the mutation results in a different binding of the inhibitor in the two complexes. In the mutant, but not in the wild-type complex, a carboxylate O atom is within hydrogen-bond distance of Thr199 Oγ1. In the wild-type enzyme ligand hydrogen bonding to this atom is normally only found for hydrogen-bond donors. The importance of this discrimination on catalysis by the enzyme is discussed briefly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444993009667
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Core-level study of WSi2 (110) (1994)
    Springer
    The European physical journal 96 (1994), S. 87-92 
    Details
    ISSN: 1434-6036
    Keywords: 73.20.-r ; 79.60.Bm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Angle resolved core level studies of the Si 2p and W 4f levels have been carried out on the (110) surface of a WSi2 single crystal using synchrotron radiation. Surface shifted components have been revealed both in the Si 2p and W 4f spectra. Investigations were carried out at two different annealing temperatures. The results indicate Si enrichment at the surface, and a larger enrichment after the higher temperature anneals. The reactivity upon initial oxygen exposure was investigated. Strong Si oxidation was observed but chemically shifted W 4f components could also be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313018
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  • 5
    Electronic Resource
    Electronic Resource
    A field study of diffusion controlled migration of copper, zinc and cadmium in a clay formation (1991)
    Springer
    Water, air & soil pollution 57-58 (1991), S. 259-268 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The distribution of Cu, Zn and Cd within an illitic clay formation located beneath a ca 150 yr old deposit of sulfidic mine tailings was studied. Total concentrations of metals have been quantified as a function of depth. The distribution between the pore water and the solid phase has been measured. Qualitative information was obtained by an operational leaching procedure. Field measurements were validated with batch adsorption experiments. The results suggest that transport of metals originating from the tailings is diffusion controlled. Apparent diffusivities as well as distribution coefficients were estimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282889
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of weathering on metal releases from an engineered deposit for alum shale leaching residues (1991)
    Springer
    Water, air & soil pollution 57-58 (1991), S. 431-440 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A pile of ca 15,000 t of crushed alum shale leaching residues from uranium refining was deposited in Ranstad, Sweden, in 1972 as a part of a pilot study of various waste storage concepts. A field study has been performed in order to evaluate the efficiency of the engineered barriers (bentoniteltill or crushed limestone) for the prevention of weathering of the leaching residues and subsequent release of metal rich leachates. The concentration levels of Fe (from pyrite in the alum shale) as well as metals associated with the pyrite (e.g. Cu and Ni) indicate that the weathering of leaching residues underneath the cover has progressed to less than 4 cm in 15 yr. No breakthrough of precipitation is indicated from the composition of the percolation water coming from the deposit. Thus, the weathering rate is reduced by ca 3 orders of magnitude in comparison with leaching residues freely exposed to air and precipitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282906
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    Paper (German National Licenses)
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