ISSN:
1432-0851
Keywords:
Key words Tumor antigens
;
Tumor immunity
;
Helper T-lymphocytes
;
Interleukin
;
Interferon
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (T-Ag) was used to immunize BALB/c mice to examine the lymphokines produced following immunization. Specifically, we examined production of interleukin-2 (IL-2), IL-4, IL-5 and interferon γ (IFNγ) from immune lymphocytes cultured with decreasing concentrations of recombinant SV40 T-Ag. We identified elevated levels of IFNγ and IL-2 by enzyme-linked immunosorbent assay and a murine CTLL-2 proliferation bioassay respectively. We were unable to detect either IL-4 or IL-5. These data indicate the previously reported tumor immunity induced by recombinant SV40 T-Ag immunization most likely reflects a TH1-like immune response based on the in vitro production of both IFNγ and IL-2 by immune lymphocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01517353
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