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  • 1
    Electronic Resource
    Electronic Resource
    Flavonoids as inducers of extracellular proteins and exopolysaccharides of Sinorhizobium fredii (1999)
    Springer
    Biology and fertility of soils 30 (1999), S. 83-89 
    Details
    ISSN: 1432-0789
    Keywords: Key words Sinorhizobium fredii ; Flavonoids ; Extracellular proteins ; Exopolysaccharides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Some flavonoids present in root exudates are inducers of nod genes in rhizobia-legume symbioses. They also induce changes in the molecular weight, structure, and level of secretion of some extracellular proteins, exopolysaccharides (EPS) and lipopolysaccharides. We showed that incubation of Sinorhizobium fredii USDA 257 with four flavonoids (genistein, naringenin, chrysin, and apigenin) promoted its growth in the late log phase. By contrast, only genistein accelerated the growth of S. fredii TU 6 under the same conditions. When both strains were incubated with naringenin the synthesis of EPS decreased. However, this compound increased the secretion of extracellular proteins in the early log phase. The specific mode of action of naringenin is still not clear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050591
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosome analysis by fluorescence in situ hybridization of callus-derived regenerants in Allium cyaneum R. (1998)
    Springer
    Plant cell reports 18 (1998), S. 209-213 
    Details
    ISSN: 1432-203X
    Keywords: Key words Somaclonal variation ; Allium cyaneum ; Fluorescence in situ hybridization ; Autotetraploid regenerants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Investigations were performed to confirm the optimal in vitro culture condition for callus induction and plant regeneration, to observe if somoclonal variation occurs among regenerated plants at the ploidy level and to analyse the chromosomal location of 5S and 18S-26S rRNA gene families using fluorescence in situ hybridization in callus-derived plants of Allium cyaneum. High-est callus initiation was achieved with bulb explants cultured on MS medium supplemented with 2,4-D and BAP at 1 mg l–1 each. A total of 195 plants was obtained when using MS medium supplemented with 1 mg l–1 NAA and 5 mg l–1 BAP; about 92% were diploid having 2n=16; 8% showed a variation in ploidy level. Using digoxigenin-labelled 5S rRNA and biotin-labelled 18S-26S rRNA gene probes, we compared the fluorescence in situ hybridization patterns of autotetraploid plants with the A. cyaneum wild type. The 5S rRNA gene sites were detected on the interstitial region in the short arm of chromosome 4 and on the interstitial region in both arms of chromosome 7. The 18S-26S rRNA gene sites were detected on the terminal region of the short arm, including the satellite of chromosome 5, as well as on a part of chromosome B. The chromosomal location of both rRNA genes in regenerated autotetraploid plants corresponded to those of the wild species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050558
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    Paper (German National Licenses)
    Fulltext
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