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  • Electronic Resource  (4)
  • 1995-1999  (4)
  • IAPS-forskolin  (2)
  • Insulin-regulated glucose transporter GLUT4  (2)
  • Biochemistry and Biotechnology  (1)
  • Engineering
  • Organic Chemistry
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  • Electronic Resource  (4)
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Year
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  • 1
    ISSN: 1432-1912
    Keywords: Glucose transport ; IAPS-forskolin ; Insulin-regulated glucose transporter GLUT4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two arginine residues (RR333/334) in the conserved GRR motif located in the endofacial loop between helix 8 and 9 of the glucose transporter GLUT4 were substituted for leucine and alanine, respectively. Reconstituted glucose transport activity of the construct (GLUT4-RR333/4LA) expressed in COS-7 or LM(TK-) cells was less than 10% of that of the wild-type GLUT4. In contrast, binding of the inhibitory ligand cytochalasin B and glucose-inhibitable photolabeling with IAPS-forskolin were not significantly affected. Exchange of a histidine residue (H337Q) previously believed to be involved in the binding of inhibitory ligands failed to affect any of the investigated parameters. These data suggest that positive charges in the GRR motif at the cytoplasmic surface of the transporter participate in the conformational changes of the carrier protein during the process of facilitated diffusion.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1912
    Keywords: Key words Glucose transport ; IAPS-forskolin ; Insulin-regulated glucose transporter GLUT4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two arginine residues (RR333/334) in the conserved GRR motif located in the endofacial loop between helix 8 and 9 of the glucose transporter GLUT4 were substituted for leucine and alanine, respectively. Reconstituted glucose transport activity of the construct (GLUT4-RR333/4LA) expressed in COS-7 or LM(TK–) cells was less than 10% of that of the wild-type GLUT4. In contrast, binding of the inhibitory ligand cytochalasin B and glucose-inhibitable photolabeling with IAPS-forskolin were not significantly affected. Exchange of a histidine residue (H337Q) previously believed to be involved in the binding of inhibitory ligands failed to affect any of the investigated parameters. These data suggest that positive charges in the GRR motif at the cytoplasmic surface of the transporter participate in the conformational changes of the carrier protein during the process of facilitated diffusion.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 22 (1998), S. 671-687 
    ISSN: 0363-9061
    Keywords: recurrent neural network ; residual soil ; shear behaviour ; simulation ; prediction ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: Modelling of shear behaviour of residual soils is difficult in that there is a significant variability in constituents and structures of the soil. A Recurrent Neural Network (RNN) is developed for modelling shear behaviour of the residual soil. The RNN model appears very effective in modelling complex soil shear behaviour, due to its feedback connections from an hidden layer to an input layer. Two architectures of the RNN model are designed for training different sets of experimental data which include strain-controlled undrained tests and stress-controlled drained tests performed on a residual Hawaiian volcanic soil. A dynamic gradient descent learning algorithm is used to train the network. By training only part of the experimental data the network establishes neural connections between stress and strain relations. Although the soil exhibited significant variations in terms of shearing behaviour, the RNN model displays a strong capability in capturing these variabilities. Both softening and hardening characteristics of the soil are well represented by the RNN model. Isotropic and anisotropic consolidation conditions are precisely reflected by the RNN model. In undrained tests, pore water pressure responses at various loading stages are simultaneously simulated. With a RNN model designed for a special drained test, the network is able to capture abrupt changes in axial and volumetric strains during shearing courses. These good agreements between the measured data and the modelling results demonstrate the desired capability of the RNN model in representing a soil behaviour. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 15 (1997), S. 9-14 
    ISSN: 0263-6484
    Keywords: endosomes ; insulin ; streptozotocin ; brush border membrane ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Chronic renal adaptation to dietary deprivation of Pi is accompanied by increased Na+/Pi co-transport across the brush border membrane of the renal proximal tubule. The increased activity of this co-transport system depends on de novo protein synthesis and insulin. The present study used normal and diabetic rats to determine if the endosomal pool of Na+/Pi co-transporters was altered by Pi deprivation and the possible role of insulin. In response to 5 days of dietary Pi deprivation there was a significant increase in endosomal Na+/Pi co-transport in control rats but there was no change in diabetic rats. The increase in endosomal Pi uptake was restored in diabetic rats treated with exogenous insulin. Na+/Pi-independent Pi uptake and proline uptake remained unchanged in all groups. The changes in endosomal Na+/Pi co-transport correlated with the abundance of the specific Na+/Pi co-transporter protein, as determined by Western blots. The pattern of endosomal changes paralleled that observed in brush border membranes. One possibility consistent with these findings is that the endosomal fraction contains newly synthesized Na+/Pi co-transporters targeted for delivery to the apical brush border membrane. Increased synthesis and delivery is required to maintain the adaptation to chronic Pi deprivation. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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