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Digitale Medien

Cyanobacteria carrying ansmt-lux transcriptional fusion as biosensors for the detection of heavy metal cations (1996)

Erbe, J L ; Adams, A C ; Taylor, K B ; [weitere]

Springer

Journal of industrial microbiology and biotechnology 17 (1996), S. 80-83

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Details

ISSN: 1476-5535

Schlagwort(e): cyanobacteria ; biosensor ; heavy metal ; lux

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract The metal-responsivesmt operator/promoter region ofSynechococcus PCC7942 was fused to theluxCDABE genes ofVibrio fischeri. Plasmid DNA (pJLE23) carrying this fusion conferred metal ion-inducible luminescence to transformed cyanobacteria.Synechococcus PCC7942 (pJLE23) was sensitive to ZnCl2 concentrations within a range of 0.5–4 μM as demonstrated by induction of luminescence. Trace levels of CuSO4, and CdCl2 were also detected.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01570047

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Digitale Medien

Ultrastructural changes in cultured human periodontal ligament cells exposed to dental materials (1995)

Adams, A. M. ; Soames, J. V. ; Searle, R. F.

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 29 (1995), S. 999-1004

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Details

ISSN: 0021-9304

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin , Technik allgemein

Notizen: The cytopathic effects of a range of dental restorative cements were examined by electron microscopy using an in vitro model with cultured test cells derived from human periodontal ligament. Monolayers were fixed and processed in situ after 2, 24, and 48 h exposure. Many cells showed lysis after two hours exposure consistent with immediate acute injury. Cultures subsequently recovered, reflecting different susceptibilities of cells to injury, and at later stages showed distinct patterns of cell damage in response to different restorative materials. These were related primarily to either cytoplasmic or nuclear damage and to changes resembling apoptosis. © 1995 John Wiley & Sons, Inc.

Zusätzliches Material: 10 Ill.