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  • Electronic Resource  (5)
  • 1995-1999  (5)
  • callus  (3)
  • growth regulators  (2)
  • Chloroplast
  • 1
    Electronic Resource
    Electronic Resource
    In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 263-268 
    Details
    ISSN: 1573-5044
    Keywords: Auxin ; callus ; cytokinin ; fennel ; histological analysis ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus induction and morphogenic response of several fennel populations were determined by genotype and hormonal treatment. 100% callus formation occurred only in the Francia Pernod population under the action of 2, 4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid and kinetin. Only this last hormonal treatment induced shoot regeneration. Plant regeneration was observed especially in Francia Pernod population. Ths calluses grown in presence of 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid plus kinetin, showed considerable differences at cytological and histological level which were correlated to their different morphogenic capability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043640
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Manganese toxicity to different growth processes in vitro in Nicotiana (1997)
    Springer
    Plant cell, tissue and organ culture 50 (1997), S. 125-129 
    Details
    ISSN: 1573-5044
    Keywords: callus ; metal tolerance ; shoot regeneration ; Solanaceae ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Manganese toxicity to germination, callus induction and shoot regeneration was studied on three cultivars of Nicotiana tabacum: BEL W3, Burley 21, Bright NC 944. All materials were cultured on MS solid medium containing 0.1 (control), 2, 5, 10, 15 and 20 mM Mn, to which 5 µM NAA and 5 µM kinetin were added for callus induction and shoot regeneration. Mn toxicity to callus growth was tested using habituated callus of Nicotiana bigelovii var. bigelovii grown on MS medium without growth regulators. Mn concentrations higher than 2 mM were toxic for germination, and concentrations higher than 5 mM were toxic for callus induction, shoot regeneration and callus growth. Among the cultivars examined, Bright tobacco appeared more tolerant to high Mn concentrations during callus formation and shoot regeneration. However, many regenerated plants capable of growing in vitro in the presence of 2 and 5 mM Mn were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005917114146
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on callus growth and morphogenesis in several species and lines of t Amaranthus (1997)
    Springer
    Plant cell, tissue and organ culture 49 (1997), S. 29-33 
    Details
    ISSN: 1573-5044
    Keywords: explant state ; genotype ; growth regulators ; organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study has been carried out on several Amaranthus L species and breeding lines (A. caudatus, A. cruentus, A. hybridus, A. hypochondriacus), some of which have previously shown poor or no plant regeneration from callusing primary explants. Callus formation occurred from explants from almost all the lines tested. Significant differences in callus growth were observed among the lines depending on the growth regulator combinations used. Cultural conditions such as type and growth regulator combination, as well as age-dependent competence of the explant tissues, also influenced the frequency of shoot (and/or root) regeneration in Amaranthus species that had been shown difficult to regenerate in the past. The results obtained demonstrate that in Amaranthus the genotype, growth regulator dose and combination, the type and the physiological stage of explant are factors of great importance for in vitro plant regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005882322044
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Response of shape in vitro cultures of shape Nicotiana tabacum L. to copper stress and selection of plants from Cu-tolerant callus (1998)
    Springer
    Plant cell, tissue and organ culture 53 (1998), S. 161-169 
    Details
    ISSN: 1573-5044
    Keywords: callus ; copper toxicity ; N. tabacum ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Nicotiana tabacum L. var. BEL W3 copper (Cu) at concentrations higher than 50 μM significantly inhibited callus growth and shoot regeneration. After 5–6 months of culture only a few morphogenic callus lines survived in the presence of 100 μM Cu. These calluses showed the capacity to grow and regenerate shoots through successive subcultures on medium containing 100 μM Cu. The 100 μM Cu-tolerant shoots, in comparison to regenerated control shoots, formed roots only when cultured in the presence of 100 μM Cu. From five independent Cu-tolerant callus lines in a culture period of 4–5 months more than 50 plants (defined ‘tolerant’) able to grow in presence of 100 μM Cu were obtained. These plants showed normal xylem tissue formation while control regenerated plants growing in normal Cu MS content (0.1 μM) had few xylem elements in the central cylinder. No difference as far chlorophyll content and chloroplast structure was found among Cu-tolerant and control plants. In Cu-tolerant plants, dry matter production was higher than in controls, particularly in roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006048031956
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Callus cultures of Artemisia absinthium L.: initiation, growth optimization and organogenesis (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 67-72 
    Details
    ISSN: 1573-5044
    Keywords: Biomass increase ; chromosome count ; growth regulators ; regeneration ; tissue culture ; wormwood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures were initiated from micropropagated Artemisia absinthium plantlets on MS basal medium supplemented with different concentrations of BA, Kn, NAA, IAA and 2,4-d in combination or singly. Supplementing the medium with low doses of both BA in combination with NAA, and Kn in combination with NAA enhanced the growth rate of callus cultures. However, cultures grew slowly following the second subculture and the majority turned brown and died within the next month. Initiation of root and shoot primordia occured directly from leaf explants cultured on 1.81 μM 2,4-d, while adventitious shoot formation from callus was observed occasionally when BA was added to the medium in combination with IAA. Furthermore, medium containing 2.22 μM BA and 2.69 μM NAA stimulated both callus growth and organogenesis on some callus cultures derived from leaves and stems of young stock material. The best results were obtained with leaf explants. Cytological analysis of root meristems revealed that all regenerants were diploid (2n=18), as expected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043430
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    Paper (German National Licenses)
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