ISSN:
1573-6903
Keywords:
Regenerating nerve
;
goldfish optic nerve
;
proteoglycans
;
heparan sulfate
;
chondroitin sulfate
;
axonal transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Structural characteristics of glycosaminoglycans (GAGs) derived from axonally transported proteoglycans (PGs) were compared in 21 day regenerating and intact goldfish optic tracts. Twenty one days following unilateral optic nerve crushes, fish received intraocular injections of35SO4. Eight hours post injection, tracts were removed and the35SO4-labeled GAGs, chondroitin sulfate (CS) and heparan sulfate (HS), isolated. The HS from regenerating optic tracts had a DEAE elution profile indicative of decreased charge density, while heparitinase treatment of HS followed by Sephadex G50 analysis of the resulting fragments showed a change in the elution pattern, suggesting reduced overall sulfation. HPLC analysis of HS disaccharides revealed a difference in the sulfation pattern of regenerating tract HS, characterized by the reduced presence of tri-sulfated disaccharides. Other structural features, such as the sizes of CS and HS, and the sulfation of CS, showed no changes during regeneration. These results indicate that changes in the structure of axonally transported HS accompany regeneration of goldfish optic axons.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00969540
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