ISSN:
0178-515X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract We investigated the effect of culture temperature on the maximum specific growth rate and the cellular sugar accumulation, and the effect of a temperature shift on the sugar accumulation of Chlorella pyrenoidosa cells in a batch culture system. Increase in temperature below 30 °C appeared to correlate with increase in the maximum specific growth rate, on the contrary the cellular sugar content showed a reverse tendency against temperature. We attempted to utilize this tendency for improving sugar productivity in Chlorella. First, we cultured Chlorella at 28 °C during the logarithmic growth phase to obtain a high specific growth rate. The culture temperature was then shifted from 28 °C to 22 °C at the late logarithmic growth phase in order to reduce the specific growth rate and enhance the cellular sugar accumulation. As a result, we obtained a 15% increase in sugar production over that obtained by cultivation at 28 °C throughout the culture. We also investigated the effect of light-dark time cycle on the sugar productivity and found that this operating variable did not affect the cellular sugar content but influenced the final cell concentration. Among the examined light-dark time cycles, maximum sugar productivity was obtained in the case of 12 h light and 12 h dark period.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004490050308
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