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  • Digitale Medien  (4)
  • 1990-1994  (4)
  • 1980-1984
  • 1965-1969
  • 1960-1964
  • 1945-1949
  • 1930-1934
  • E. coli  (2)
  • calcium  (2)
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  • Digitale Medien  (4)
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  • 1990-1994  (4)
  • 1980-1984
  • 1965-1969
  • 1960-1964
  • 1945-1949
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Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 135 (1991), S. 185-196 
    ISSN: 1573-5036
    Schlagwort(e): calcium ; critical phosphorus concentration ; gypsum ; lime ; perennial ryegrass ; root membranes ; soluble carbohydrate ; zinc
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The results of a previous study had suggested that under conditions of limited P availability, Ca may be able to compensate for P in the shoot tissue of perennial ryegrass. To verify this preliminary finding, a factorial experiment was set up which simultaneously tested the effects of Ca and P fertilization on the yield and chemical composition of perennial ryegrass. Calcium was supplied as either lime or gypsum in order to differentiate between the effects of Ca and pH on the response of perennial ryegrass to P fertilization. In the final stage of the experiment a Zn treatment was included, to see whether altering the P/Zn ratios of plant shoots had any influence on the purported interaction between Ca and P. The results demonstrated that the P-sparing effect of lime occurs, at least partly, because Ca application improves the efficiency of absorbed P for DM production. However, it was reasonably clear that the site of the interaction between Ca and P was the soil-root interface, and not shoot tissue. It was suggested that under conditions of limited P supply, Ca stablizes root membranes and thereby minimizes both the efflux losses of nutrients from root tissue, and the compensatory flow of photosynthates from shoots to roots. No interaction was observed between P and Zn treatments in this study. Instead, a positive interaction was found between lime and Zn treatments, which suggests that the stabilizing action of Ca on root membranes requires Zn as a co-stabilizing factor. It is proposed that chemical analysis of shoot tissue alone may not be sufficient to accurately diagnose the P, Ca or Zn status of whole plants, since the critical levels of these elements in shoots appear to bear little relation to their requirements in the rhizosphere.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 143 (1992), S. 19-31 
    ISSN: 1573-5036
    Schlagwort(e): ammonium ; calcium ; carboxylate/organic nitrogen ratio ; 15N isotope ; nitrate ; nitrogen cycling ; perennial ryegrass
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Solution culture studies have shown that plant uptake of NH4 + and NO3 - can be improved by increasing the concentration of Ca2+ in the root environment: the same may be true for grass grown in soil culture. An experiment was set up to see whether gypsum (CaSO4 2H2O) increased the rate at which perennial ryegrass absorbed 15NH4 + and 15NO3 - from soil. The results demonstrated that gypsum increases the rates of uptake of both NH4 + and NO3 - by perennial ryegrass. However because there was little potential for mineral-N loss from the experimental system, either by gaseous emission or by N immobilization, long term improvements in fertilizer efficiency were not observed. Nitrogen cycling from shoots to roots commenced once net uptake of N into plants had ceased. Labelled N transferred thus to roots underwent isotopic exchange with unlabelled soil N. It was suggested that this exchange of N might constitute an energy drain from the plant, if plant organic N was exchanged for soil inorganic N. The fact that the exchange occurred at all cast doubt on the suitability of the 15N-isotope dilution technique for assessing fertilizer efficiency in medium to long term experiments. There was evidence that the ‘extra’ NO3 --N taken up by plants on the all-nitrate treatments as a result of gypsum application, was reduced in root tissue rather than in shoots, but to the detriment of subsequent root growth and N uptake.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 1318-1324 
    ISSN: 0006-3592
    Schlagwort(e): glucose metabolism ; pet operon ; E. coli ; fermentation ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The glucose metabolism of an Escherichia coli strain bearing mutations abolishing both acetyl phosphotransferase (PTA) and acetate kinase (ACK) activities was studied under aerobic and anaerobic conditions. These studies were conducted in a complex medium with the mutant carrying no plasmid, the mutant carrying the common cloning vector pUC19, and the mutant carrying a plasmid bearing the “pet” operon that encodes Zymomonas mobilis pyruvate decarboxylase and alcohol dehydrogenase activities. The mutant carrying no plasmid showed lower specific growth and glucose uptake rates relative to the parent wild-type strain (K-12), Lactic acid was produced at higher levels than the wild type, and considerable amounts of pyruvic acid were secreted as an unusual byproduct. Analysis of other fermentation products showed low but significant amounts of acetic acid, no accumulation of formic acid, and lower secretion of succinate and ethanol. The maintenance of the plasmid pUC19 in the mutant negatively affected metabolism. Expression of the pet operon overcame the metabolic stress caused by the plasmid, enhancing growth and glucose uptake rates to the values observed in the plasmidfree mutant. Also, expression of the pet operon allowed consumption of pyruvate accumulated during the first hours of fermentation.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 39 (1992), S. 59-65 
    ISSN: 0006-3592
    Schlagwort(e): pet operon ; E. coli ; metabolic fluxes ; metabolic engineering ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Fermentation patterns of Escherichia coli HB101 carrying plasmids expressing cloned genes of Zymomonas mobilis pyruvate decarboxylase (PDC) and alcohol dehydrogenase li (ADH) were determined in glucose-limited complex medium in pH-controlled anaerobic batch cultivations. Time profiles of glucose, dry cell weight, succinate, formate, acetate, and ethanol were determined, as were the activities of ADH and PDC. Fluxes through the central carbon pathways were calculated for each construct utilizing exponential phase data on extracellular components and assuming quasi-steady state for intermediate metabolites. Overall biomass yields were greatest for cells expressing both PDC and ADH activities. Yields of carbon catabolite end products were similar for all PDC-expressing strains and different from those for other strains. Relative to its glucose uptake rate, the strain with greatest PDC and ADH activities produces formate and acetate more slowly and ethanol more rapidly than other strains. Strong influences of plasmid presence and metabolic coupling complicate detailed interpretations of the data.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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