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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 5 (1976), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Soluble blood group substances, with human H and A activity, have previously been shown to block the interaction between guinea pig peritoneal macrophages and migration inhibition factor from fetal calf serum (FCS-MIF), Conversely, incubation of macrophapes at 37°C for 1 h in the presence of 0.1% blood group substance, followed by thorough washing, potentiates the action of FCS-MIF. The sensitivity of the macrophages is markedly increased, allowing detection of subthreshold levels of FCS-MIF. Blood group substances (BGS) labeled with radioidine are taken up by macrophages, and a proportion remains on the surface. This radiolabeled BGS is lost from the surface spontaneously, and the rate of loss is increased by treatment with trypsin. It is suggested that the BGS mimic the natural macrophage receptor for FCS-MIF and potentiate its effect by incorporating new receptors into the macrophage membrane.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Guinea pig peritoneal exudate cells were harvested 3 to 4 days after the intra-peritoneal injection of Marcol oil. The washed cells were exposed to various concentrations of sodium periodate in phosphate-buffered saline (PBS) at pH 7.4 for 10 min at +4°C. The cells were then used in the in vitro migration assay, and migration was consistently inhibited at concentrations from 10−3 to 10−5M. The viability of the macrophages was not affected by this treatment. Sodium borohydride (10−3 to 10−5M) in PBS for 10 min at pH 7.4 reversed the periodate effect. Experiments with purified macrophages showed that sodium periodate has a direct effect on macrophage function rather than an indirect effect via the potentiation of migration inhibition factor. In support of this, the in vitro spreading of macrophages on glass substrate foe 1 h has been shown to be inhibited. This spreading inhibition can also be reversed by treatment with sodium borohydride. These results provide a new approach to understanding the biological significance and role of macrophage migration inhibition.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 41 (1992), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The powdery mildew Erysiphe pisi var. pisi, complete with its teleomorph, has been observed on faba beans (Vicia faba cultivars) for the first time in Britain. It appeared in a glasshouse on Syrian lines of fodder bean and English cultivars of broad bean. It also infected tare (Vicia sativa) and sweet pea (Lathyrus odoratus), but not edible pea (Pisum sativum), ornamental lupin (Lupinus polyphyllus), French bean (Phaseolus vulgaris) or sainfoin (Onobrychis viciaefolia). Inoculations in a glasshouse showed that another strain of E. pisi var. pisi found on outdoor everlasting pea (Lathyrus latifolius) was also capable of affecting faba bean, and was distinguished by its ability to infect edible pea. A third strain found on outdoor ornamental lupin infected only lupin. E. pisi var. pisi was shown to be morphologically and pathogenically distinct from the Erysiphe sp. newly affecting tomatoes in Britain.
    Type of Medium: Electronic Resource
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  • 14
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 41 (1992), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The incidence of potato pathogens on healthy roots of micropropagated (MP) and seed tuber (ST) plants was examined on successive dates during the growing season in two field experiments. Microplants were grown in a glasshouse for 4–5 weeks in perlite or peal-based substrates, and exposed or not to natural inoculum before planting in the field. The seed tubers originated from stocks of visually clean or moderately blemished tubers and were surface-sterilized or not before planting. Polyscytalum pustulans and Helminthosporium solani only infected roots of ST plants and inoculated MP plants. The incidence of P. pustulans was affected by seed tuber-borne inoculum and, in I year, by the substrate. H. solani was detected infrequently on roots. Rhizoctonia solani was present at low frequencies in most root samples, and more ST than MP plant roots were colonized; there were no substrate effects. In 1 year, increased inoculum levels increased root infection, but only in MP roots. Colletotrichum coccodes occurred at high frequencies and was most common in roots of ST plants. Progeny tubers showed some treatment effects when tested in September and after storage for 6 months, but there were no consistent relationships between root and progeny tuber infection.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 8 (1978), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Leucocyte migration in the presence and absence of a soluble lung homogenate has been studied in a group of asymptomatic cigarette smokers. Results were expressed as migration indices. Controls consisted of age-matched subjects who had never smoked and had no past history of any pulmonary disease. The migration indices, the number of pack-years* smoked and the results of pulmonary function studies were tested for correlation.The mean leucocyte migration index of the test group of sixteen smokers was significantly less than was that of the control group (P 〈 0·001). Inhibition of leucocyte migration in the presence of the lung homogenate was not observed in any of the control subjects. Six of the sixteen asymptomatic cigarette smokers, however, were identified by inhibition of leucocyte migration in the presence of the lung homogenate. The number of pack-years which had been smoked did not correlate with the migration indices (r=−0·06); however, inhibition of leucocyte migration was not observed in subjects who had smoked less than 6 pack-years.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 7 (1977), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Lung-specific, cellular hypersensitivity was studied in patients with chronic pulmonary diseases. To do this, a leucocyte migration inhibition assay was performed using patient and control leucocytes incubated in the presence and absence of a soluble lung extract. Control antigens consisted of liver and kidney extracts. The immunological reactivity of these control antigens was tested by measuring migration, subsequent to incubation of leucocytes from patients with hepatic or renal disease with the liver and kidney extracts, respectively. As a second in vitro test of cell-mediated immunity, a lymphocyte cytotoxicity assay was performed, using as target cells normal chromium-labelled human lung cells.Leucocyte migration inhibition was produced by the lung extract in four out of six patients with chronic bronchitis, five out of seven patients with tuberculosis, one out of five patients with sarcoidosis and in the single patient studied with rheumatoid lung disease. Inhibition did not occur with the leucocytes from control subjects. Neither was migration inhibited when leucocytes from the patients with lung disease were incubated with the control antigens. The reactivity of these control antigens was confirmed by the fact that leucocyte inhibition occurred in two out of three patients with hepatic disease, studied using the liver extract, and in two out of three patients with renal disease, studied with the kidney extract. The cytotoxic potential of anti-lung lymphocytes was demonstrated by the fact that significant target cell cytotoxicity occurred using lymphocytes from two out of four patients with chronic bronchitis, four out of five patients with tuberculosis and in two out of four patients with sarcoidosis.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Adult fleas, spent and unspent culture media were extracted and the radio-atlergosor-bent test (RAST) performed with sera of 48 cat flea skin test-positive individuals from the Tampa Bay area of Florida. Sixteen sera (33.6%) had a positive RAST to the cat flea extract prepared in our laboratory [1.7-11.4% of the total counts (TC) added]. Six of the 16 sera (12.5%) also contained specific IgE to allergens in thespent medium (0.8-3.3% TC). The allergen composition and strength were studied by RAST inhibition of two commercial cat flea extracts and compared with our in-house flea extract. The results demonstrated similar allergen compositions and different potencies. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the in-house flea extract showed several Coomassie blue-stained bands (10–85 kD). SDS-PAGE immunoblols revealed five IgE-binding bands at 34, 35, 39, 54 and 60 kD. Flea allergens were quantified in eight house dust samples using RAST inhibition assays and expressed as RAST inhibition units; five of these samples contained detectable levels. Cat flea allergens may contribute to the allergenicity of house dust in areas of heavy llea infestation.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 34 (1978), S. 3719-3722 
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 312 (1978), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 20
    ISSN: 1432-0649
    Keywords: 42.55.Px ; 32.30.Jc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Saturated-absorption signals on the calcium 657 nm transition are observed by direct absorption using diode lasers and a high flux atomic-beam cell. Line-widths as narrow as 65 kHz are observed with a high signal-to-noise ratio. Prospects for using this system as a compact wavelength/frequency reference are considered.
    Type of Medium: Electronic Resource
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