ISSN:
1434-0879
Keywords:
Chemosensitivity test
;
Collagen gel matrix
;
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
;
Human transitional cell carcinoma
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An organ culture system using collagen gel matrix (CGM) was modified and established successfully as a rapid and convenient method for determination of anti-cancer drug sensitivities. The cell viability of a tumor fragment was measured by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to a colored formazan product, which allowed for quantitative and simple analysis. The sensitivities of KK-47 bladder tumor from nude mice to various anti-cancer drugs tested corresponded closely to those determined in the originial CGM assay system using tritiated thymidine, which has a high clinical correlation. Our modified method can be used as a practical and highly reproducible chemosensitivity test in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01788824
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