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The VirD2 protein of Agrobacterlum tumefaciens carries nuclear localization signals important for transfer of T-DNA to plants (1993)

Rossi, L. ; Hohn, B. ; Tinland, B.

Springer

Molecular genetics and genomics 239 (1993), S. 345-353

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ISSN: 1617-4623

Keywords: β-Glucuronidase ; Transient expression ; Ti plasmid ; Nuclear localization signal ; Tobacco seedling

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Agrobacterium tumefaciens is able to transfer a piece of DNA, the T-DNA, to the nucleus of the plant cell. The VirD2 protein is required for the production of the T-DNA, it is tightly linked to the T-DNA and it is thought to direct it to the plant genome. Two nuclear localization signals (NLS), one in the N-terminal part and one in the C-terminal part of the VirD2 protein, have been shown to be able to target marker proteins to the plant nucleus. Here we analyze nuclear entry of the T-DNA complex using a new and very sensitive assay for T-DNA transfer. We show that optimal T-DNA transfer requires the VirD2 NLS located in the C-terminal part of the protein, whereas mutations in the N-terminal NLS coding sequence seem to have no effect on T-DNA transfer.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00276932

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Relation between hyaluronan and sulphated glycosaminoglycan synthesis and degradation in cultured embryonic fibroblasts. Effect of concanavalin a and ammonium chloride administration (1991)

Carinci, P. ; Locci, P. ; Evangelisti, R. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 9 (1991), S. 255-262

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ISSN: 0263-6484

Keywords: Hyaluronan ; glycosaminoglycans ; GAG synthesis ; GAG degradation ; chick embryo ; fibroblasts ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: In order to evaluate the relationship between glycosaminoglycan (GAG) synthesis and degradation, the effect of NH4Cl, which inhibits lysosomal degradation, on GAG production was analysed in vitro in concanavalin A (Con A) stimulated fibroblasts from 7 and 14-day-old chick embryos. 35SO4 incorporation into total proteoglycan (PG), 3H incorporation into individual GAG classes, β-N-acetyl-D-glucosaminidase and β-D-glucuronidase activity were determined. The results indicate a correlation between Con A and NH4Cl effects: NH4Cl induced a reduction principally in the GAG classes most stimulated by Con A. Thus HA and DS are much more stimulated by Con A and inhibited by NH4Cl than are CS and HS.

Additional Material: 2 Ill.