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  • 1
    Electronic Resource
    Electronic Resource
    Electrotransformation of whole cells of Brevibacterium sp. R312 a nitrile hydratase producing strain: Construction of a cloning vector (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 81 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: A rapid and effective method is described for electroporation of Brevibacterium sp. R312, a coryneform strain producing nitrile hydratase and amidase. The transformation efficiency of the method is 108 transformants per μg of plasmid under optimal conditions. Parameters optimised included field strength (11.8 kV cm−1), pulse length (2.4 ms), plasmid DNA concentration (0.25 μg ml−1 and cell density (1010 cells ml−1). Surprisingly, the transformation efficiency did not vary with the growth stage, in contrast to results in the literature. A shuttle vector was constructed containing several unique cloning sites down-stream of the SP6 RNA polymerase promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04743.x
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  • 2
    Electronic Resource
    Electronic Resource
    Selection and study of a Candida molischiana mutant derepressed for β-glucosidase production (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 118 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A mutant strain of Candida molischiana was selected. Analysis of the exocellular activity of Candida molischiana 35M5N grown on different carbon sources revealed that the biosynthesis of β-glucosidase is derepressed in this yeast strain. The strain is not a hyper-producer mutant. There were no observed differences in the endocellular and parietal activities of the wild and mutant strains. However, the mutant strain produced 35-fold more enzyme than the wild-type in the culture medium with glucose as carbon source. When glucose was used as carbon source, the mutant strain produced 90% more exocellular enzyme than when cellobiose was used as the carbon source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06829.x
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning of the wide spectrum amidase gene from Brevibacterium sp. R312 by genetic complementation. Overexpression in Brevibacterium sp. and Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 122 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The amiE gene of Brevibacterium sp. R312 encoding wide spectrum amidase was isolated by complementation of a Brevibacterium sp. mutant using a plasmid gene bank of chromosomal DNA. The amiE structural gene and its promoter were localized on a 1.8-kb fragment by subsequent subcloning and complementation studies. In Brevibacterium sp., the investigation of amidase activities related to one copy of the gene suggested that the regulation of the amiE gene expression was under negative control. High expression levels have been obtained in Brevibacterium sp. and, after substitution of the amiE promoter by the tac promoter, in Escherichia coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07155.x
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  • 4
    Electronic Resource
    Electronic Resource
    Detoxification of cassava pulp using Brevibacterium sp. R312 (1990)
    Springer
    Applied microbiology and biotechnology 33 (1990), S. 529-533 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Brevibacterium sp. strain R312 has an endocellular β-glucosidase, a nitrile hydratase and an amidase that can break down some cyanoglucosides. Nonsterile cassava pulp suspensions were fermented using this strain and 70%–80% reduction of nitrile compounds, in particular cyanoglucosides and α-hydroxynitriles, was observed. This type of nitrile-hydratase-active microorganism could be a solution for the detoxification of cassava. Experiments conducted with the yeast Candida molischiana and C. wickkerhamii showed no improvement in detoxification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172546
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  • 5
    Electronic Resource
    Electronic Resource
    Correlations between theophylline concentrations in plasma, erythrocytes and cantharides-induced blister fluid and peak expiratory flow in asthma patients (1992)
    Springer
    European journal of clinical pharmacology 43 (1992), S. 563-565 
    Details
    ISSN: 1432-1041
    Keywords: Theophylline, Asthma ; blister fluid, erythrocytes, free levels, peak expiratory flow, pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Theophylline levels in plasma, erythrocytes and cantharides-induced blister fluid (BF) have been correlated with peak expiratory flow (PEF) in asthmatic patients. Nine asthmatic men received an IV infusion of 240 mg theophylline at a constant rate for 30 min. The theophylline concentrations in erythrocytes and blister fluid were 37 % and 78 % of those in plasma. There was not a strong correlation between erythrocyte or blister fluid penetration and the clinical effect of theophylline.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02285104
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  • 6
    Electronic Resource
    Electronic Resource
    Posterior approach to the rectum for treatment of selected benign lesions (1991)
    Springer
    International journal of colorectal disease 6 (1991), S. 100-102 
    Details
    ISSN: 1432-1262
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'accès des lésions du rectum moyen peut être difficile. Ce travail résume notre expérience d'un abord postérieur du rectum chez 22 hommes et 13 femmes d'une âge de 21 à 96 ans. Les indications chirurgicales comprennent des tumeurs villeuses, des prolapsus du rectum, des sténoses rectales et des fistules du rectum. Aucune complication post-opératoire ne fut observée chez 20 malades, toutefois une fistule se developpa chez 7 patients dont 3 durent être traités par colostomie proximale et cure chirurgicale. 4 fistules guérirent spontanément. 2 malades ont developpés une éventration périnéale. L'examen anatomopathologique des tumeurs villeuses a montré une infiltration néoplasique dans trois cas nécessitant une résection rectale avec une anastomose coloanale termino-terminale. Chez 2 malades une incontinence légère est apparue traitée par Biofeedback. Une douleur périnéale résiduelle est rapportée par 2 malades. L'abord postérieur du rectum est particulièrement utile pour les lésions bénignes trop hautes pour une exérèse trans anale et trop basse pour une résection abdominale.
    Notes: Abstract Access to lesions in the mid-rectum can be difficult. This report summarizes our experience with a posterior approach to the rectum in 22 men and 13 women, age range 21 to 96 years. Surgical indications included villous tumours, rectal prolapse, rectal strictures or rectal fistulae. No postoperative complications were observed in 20 patients, but fistulae developed in seven patients, of whom three required proximal colostomy and surgical treatment. Four healed spontaneously. Two patients developed sacrococcygeal hernia. Pathologic examination of villous tumour showed extensive malignant change in three cases requiring rectal resection with end-to-end colo-anal anastomosis. In two patients mild incontinence developed, treated by biofeedback. Residual peri-anal pain was reported by two patients. The posterior approach to the rectum is particularly useful for benign lesions too high for a transanal resection and too low for a transabdominal resection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300203
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  • 7
    Electronic Resource
    Electronic Resource
    N-terminal amino acid sequence of mutant strainBrevibacterium sp. adipamidase (1993)
    Springer
    Antonie van Leeuwenhoek 64 (1993), S. 35-38 
    Details
    ISSN: 1572-9699
    Keywords: amidase-amino acid sequences ; Brevibacterium ; dinitriles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adipamidase of a mutant strainBrevibacterium sp. R312 involved in the degradation of adiponitrile to adipic acid was purified. Its N-terminal amino acid sequence was shown to be identical toBrevibacterium sp. R312 enantio-selective amidase andRhodococcus sp. N-774 amidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00870919
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  • 8
    Electronic Resource
    Electronic Resource
    Natural nitriles and their metabolism (1990)
    Springer
    World journal of microbiology and biotechnology 6 (1990), S. 83-108 
    Details
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé Ce travail passe en revue les nombreux composés nitrillques qui ont été isolés du royaume des plantes et animaux. Deux types de molécules à potentiel toxique sont largement répandus, notamment les glycosides cyanogènes et les cyanolipides. De nombreux autres nitriles aromatiques et alystatiques sont synthétisés dans une moindre mesure. Diverses études sur la synthèse et la dégradation de ces composés cyanogenes sont également passés en revue afin de mettre en évidence l'emploi putalli des différents microorganismes pour la detoxification de denrées et matières alimentalres.
    Notes: Abstract The present work reviews the numerous nitrile compounds that have been isolated from plants and animals. Two kinds of potentially toxic molecules are widespread, namely the cyanogenic glycosides and cyanollpids. Many other aromatic and allphatic nitriles are synthesized to a lesser extent. Different studies on the synthesis and degradation of these cyanogenic compounds are also reviewed to emphasize the potential use of different microorganisms for the detoxification of food and foodstuff.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01200927
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  • 9
    Electronic Resource
    Electronic Resource
    Use of brevibacterium sp 19-derived amidase for the synthesis of hydroxamic fatty acids (1990)
    Springer
    Biotechnology letters 12 (1990), S. 689-692 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Oleylhydroxamic acid (Oleyl HA) was produced by acylation of neutralized hydroxylamine with oleic acid in buffered or in solvent media using the broad-spectrum amidase activity ofBrevibacterium (BB) sp 19 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01088195
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  • 10
    Electronic Resource
    Electronic Resource
    Evidence for the β-glucosidase activity and cellobiose fermentation by various kloeckera strains (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 451-457 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Twenty one yeast strains were tested for their β-glucosidase activity. Only four produced significant quantities of β-glucosidase but none of them was able to secrete it. These four strains were tested under fermentative conditions and none of them was able to ferment cellobiose anaerobically.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100514
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