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  • Electronic Resource  (3)
  • 1990-1994  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Are similar inflammatory factors involved in strenuous exercise and sepsis? (1994)
    Springer
    Intensive care medicine 20 (1994), S. 602-610 
    Details
    ISSN: 1432-1238
    Keywords: Exercise ; Leukocyte ; Inflammatory response ; Cytokine ; Endotoxin ; Myeloperoxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An increasing body of data suggest that strenuous exercise triggers an inflammatory response having some similarity with those occurring in sepsis. Indices of this inflammatory response to exercise (IRE) especially include leukocytosis, release of inflammatory mediators and acute phase reactants, tissue damage, priming of various white blood cell lines, production of free radicals; activation of complement, coagulation and fibrinolytic cascades. Inflammatory responses to strenuous exercise and sepsis could in part be due to the release of endotoxin in blood as common triggering factor, but it seems that tissue damage and/or contact system activation are more important triggering mechanisms in exercising subjects. While the magnitude and duration of cellular and humoral changes associated with IRE are quite different from those observed in sepsis, recent human studies suggested that chronic and/or excessive IRE could have adverse effects. Among the possible consequences of acute and chronic IRE are delayed onset muscular soreness and loss of force, cardiovascular complications, intravascular hemolysis, hypoferraemia and increased susceptibility to infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01705731
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Self-labeling of human polymorphonuclear leucocyte myeloperoxidase with125iodine (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 952-957 
    Details
    ISSN: 1420-9071
    Keywords: Human ; leucocytes ; myeloperoxidase-iodination-radioimmunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In order to obtain a radioimmunoassay (RIA) technique for the measurement of human plasma myeloperoxidase (MPO), we purified the enzyme from polymorphonuclear granulocytes (neutrophils), and compared three methods of labeling it with125Iodine: chloramine T, lactoperoxidase, and an original technique of ‘self labeling’ based on the ability of the enzyme to oxidize and bind125I in the presence of H2O2. The chloramine T technique produced a degraded protein, as well shown by a high non-specific binding of tracer to antibody. The lactoperoxidase technique did not succeed in labeling MPO with an adequate specific activity. In contrast, the self-labeling method gave a stable tracer with a specific activity of 23 μCi/gmg MPO (85 MBq), a satisfactory level of immunoreactivity, and a low-specific binding (≤3%). After labeling, purification of tracer was achieved by gel filtration chromatography in phosphate buffer (0.05 M; pH7) to which 0.1% poly-L-lysine was added. The labeled molecule remained stable for 40 days and could be used for RIA with a polyclonal antibody raised in rabbits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01929890
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Exercise induces pentane production and neutrophil activation in humans. Effect of propranolol (1990)
    Springer
    European journal of applied physiology 61 (1990), S. 319-322 
    Details
    ISSN: 1439-6327
    Keywords: Exercise ; Pentane ; β-Adrenergic blockade ; Myeloperoxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of β-adrenergic receptor blockade on exercise-induced lipid peroxidation in man has been examined by measuring the production of pentane in expired air. For this purpose, five healthy male subjects were subjected to dynamic exercise of graded intensity on a cycle ergometer (10 min at 45%, 5 min at 60% and 75% maximal oxygen uptake 1 h after ingestion of either a placebo or 40-mg propranolol. At rest, mean pentane concentration ([pent]) with placebo was 4.13 pmol · l−1, SD 2.14. After exercise, this value significantly increased by 310% (17.1 pmol · l−1, SD 7.73, P 〈 0.01). Oral administration of 40-mg propranolol significantly lowered the mean resting [pent] to 1.75 pmol · l−1, SD 0.77, P 〈 0.05. After exercise, the increase of [pent] was much smaller (240%) and was less significant (P 〈 0.2) than with the placebo. The mechanism of this inhibitory effect of propranolol remains to be elucidated. However, as indicated by the measurement of plasma myeloperoxidase concentration, it can be concluded that the antioxidant property of propranolol cannot be attributed to the inhibition of neutrophil activation, a possible source of free radicals during exercise.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00357620
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    Paper (German National Licenses)
    Fulltext
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