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  • Electronic Resource  (5)
  • 1985-1989  (2)
  • 1980-1984  (3)
  • 1900-1904
  • Ultrastructure  (3)
  • Azolla  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 389 (1980), S. 1-11 
    ISSN: 1432-2307
    Keywords: Soft tissue sarcoma ; Myofibroblasts ; Ultrastructure ; Host response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 129 soft tissue sarcomas was examined ultrastructurally to determine in which neoplasms and to what extent myofibroblasts could be demonstrated. Twenty cases of fibromatosis and fasciitis served as controls. Myofibroblasts were identified in all 30 cases of malignant fibrous histiocytoma and all 4 cases of well-differentiated sclerosing liposarcoma. Though most numerous in areas of desmoplasia, in no instance did myofibroblasts constitute the dominant cellular constituent of either neoplasm. Myofibroblasts were identified with lesser frequency and in smaller numbers in fibrosarcoma, synovial sarcoma, malignant hemangiopericytoma and neuroblastoma. None were observed in a wide assortment of diverse sarcomas in which desmoplasia was not a feature. In comparison each lesion judged by light microscopy to represent either fibromatosis or fasciitis was composed principally of myofibroblasts. The demonstration of abundant myofibroblasts within a soft tissue lesion which has been subjected to wide sampling strongly suggests a benign proliferative process as opposed to a malignant neoplasm. It is hypothesized that myofibroblasts observed within collagenized regions of soft tissue sarcomas may constitute an expression of host response to neoplasia.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 385 (1980), S. 283-291 
    ISSN: 1432-2307
    Keywords: Hodgkin's disease ; Myofibroblasts ; Ultrastructure ; Nodular sclerosis ; Host response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ten cases of nodular sclerosing Hodgkin's disease involving lymph nodes were studied by electron microscopy to determine the ultrastructural composition of the nodule-stromal interphase and the collagenized regions. In addition to a few lymphocytes, rare eosinophils and neutrophils, abundant filamentous and granular electron dense material, collagen fibers and myofibroblasts were observed in all instances. Since myofibroblasts possess contractile and synthetic properties, it is likely they contribute to the retraction and sclerosis which together represent one of the morphologic hallmarks of the disease. The dense fibrosis and contractile state of such tissue may constitute a beneficial host response to contain and limit local and vascular invasion by the neoplastic cellular population, thus contributing to the relative benignity of this form of Hodgkin's disease.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 43 (1989), S. 223-232 
    ISSN: 1573-5060
    Keywords: Azolla ; classification ; fingerprinting ; isozymes ; taxonomy ; trichomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Azolla accessions (section Azolla) from the germplasm collections of the International Rice Research Institute and Washington State University were fingerprinted and classified by enzyme electrophoresis and leaf trichome morphology. A. filiculoides was enzymatically distinctive and also reliably identified by its prominent one-celled trichomes. Neotropical accessions labelled as A. filiculoides proved to be members of other species. Two groups of isolates were designated A. rubra, but those from Japan were identified as A. filiculoides. The A. rubra of Australia-New Zealand was biochemically unique and possessed less protuberant trichomes than A. filiculoides. A. microphylla, A. mexicana, and A. caroliniana were phenetically similar, but a. microphylla was identifiable from the others in the banding patterns of certain enzymes. A. mexicana and A. caroliniana were closely related enzymatically. The two-celled leaf trichomes of these three species were similar in size and shape.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 42 (1989), S. 163-170 
    ISSN: 1573-5060
    Keywords: Azolla ; fern ; isozymes ; electrophoresis ; biochemical taxonomy ; differentiation ; variation ; hybrid identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Enzyme electrophoresis was used to differentiate sections within the genus Azolla Lam. and demonstrate the value of this method in fingerprinting taxa. Polyacrylamide gel methodology has been developed to identify suspected species of this genus. Preliminary examination of selected isolates indicated that allozyme diversity was particularly evident in section Azolla, where specific classification by morphological means is difficult. Leaf tissue was preferable to root tissue for this purpose because of the ease of tissue preparation and the superior resolution and staining intensity of enzymes. Contribution of enzyme bands by the cyanobiont to zymograms was not significant.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0878
    Keywords: Myocardium ; Ultrastructure ; Myocardial biopsy ; Morphometry ; Papillary muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The papillary muscle of the cat heart's right ventricle has not been studied previously with quantitative ultrastructural techniques despite its wide use for functional studies. This tissue was perfusion-fixed, processed for electron microscopy, and morphometric techniques were used to assess the ultrastructural characteristics of the papillary muscle as well as the working myocardial cells. The results of this study were that 73.5% of the papillary muscle was composed of muscle cells, 9.7% of blood vessels, and the remainder of interstitial connective tissue. In the muscle cell the volume fraction of mitochondria was 17.3%, that of myofibrils was 49.8%, and that of the nucleus was 2.0%. The mitochondria to myofibrils ratio was 0.36 and the surface to volume ratio was 0.309. In a quantitative ultrastructural comparison of perfusion and immersion fixed tissue it was found that significant differences in the volume density of the blood vessel lumen existed between the two groups. In addition, there were significant differences in the volume fraction of mitochondria and nucleus between perfusion-fixed and immersion-fixed muscle cells. A concurrent significant decrease between the two groups was also found for the ratio of mitochondria to myofibrils. The perfusion-fixed tissue can be considered to provide only normal baseline data for the papillary muscle of the right ventricle. These data are important as they can be used in future structure-function studies on normal and pathological heart tissue.
    Type of Medium: Electronic Resource
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