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  • Electronic Resource  (11)
  • 1985-1989  (4)
  • 1975-1979  (7)
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  • Electronic Resource  (11)
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  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of division in PHA-stimulated pig lymphocytes (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 30-31 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Our results indicate that pig lymphocytes in culture complete their 1st division at 24 h. At 36 h there are 9% of cells in 2nd division. 3rd mitosis appears at 48 h; and at 72 h there are cells engaged in the 4th division.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01921882
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  • 2
    Electronic Resource
    Electronic Resource
    A technique for chromosome analysis of small rodents without sacrificing the animal (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 698-699 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Resumen Se describe una técnica que hace posible el estudio cromosómico de pequeños roedores sin sacrificar el animal. El método consiste en: a) se introduce una microcámara de difusión cargada con un antígeno en la cavidad abdominal del animal a estudiar, b) se extrae la microcámara cuando está rodeada por una población de células inmunocompetentes en división activa (el animal se colchiniza 3 h antes de sacar la microcámara), c) se colectan las células adheridas a la cámara mediante tratamiento enzimático con Pronasa, d) se preparan las metafases para estudio cromosómico, mediante shock hipotónico, fijación y preparación de extendidos por secado al aire.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01944637
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The pattern of restriction enzyme-induced banding in the chromosomes of chimpanzee, gorilla, and orangutan and its evolutionary significance (1985)
    Springer
    Journal of molecular evolution 22 (1985), S. 323-333 
    Details
    ISSN: 1432-1432
    Keywords: Pongidae ; Chromosome ; Karyotype ; Restriction enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pattern of banding induced by five restriction enzymes in the chromosome complement of chimpanzee, gorilla, and orangutan is described and compared with that of humans. The G banding pattern induced by Hae III was the only feature common to the four species. Although hominid species show almost complete chromosomal homology, the restriction enzyme C banding pattern differed among the species studied. Hinf I did not induce banding in chimpanzee chromosomes, and Rsa I did not elicit banding in chimpanzee and orangutan chromosomes. Equivalent amounts of similar satellite DNA fractions located in homologous chromosomes from different species or in nonhomologous chromosomes from the same species showed different banding patterns with identical restriction enzymes. The great variability in frequency of restriction sites observed between homologous chromosome regions may have resulted from the divergence of primordial sequences changing the frequency of restriction sites for each species and for each chromosomal pair. A total of 30 patterns of banding were found informative for analysis of the hominid geneaalogical tree. Using the principle of maximum parsimony, our data support a branching order in which the chimpanzee is more closely related to the gorilla than to the human.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02115688
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  • 4
    Electronic Resource
    Electronic Resource
    Deuteron photodisintegration between 100 MeV and 255 MeV (1986)
    Springer
    Czechoslovak journal of physics 36 (1986), S. 290-295 
    Details
    ISSN: 1572-9486
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01597161
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  • 5
    Electronic Resource
    Electronic Resource
    Karyological conservatism in South American camelids (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 622-624 
    Details
    ISSN: 1420-9071
    Keywords: Karyological conservatism ; camelids ; camelids karyology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Llama, guanaco, vicuna and alpaca show similar diploid numbers, gross chromosomal morphology and homologous G, C and NOR banding patterns. This chromosomal homology is also found in the two-humped camel and very probably in the one-humped camel as well. These findings indicate that the camelid karyotype can probably be traced back to early Miocene times. This probably represents the most extreme case of chromosomal conservatism among mammals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01955563
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  • 6
    Electronic Resource
    Electronic Resource
    Kinetics of lymphocyte division in blood cultures studied with the BrdU-giemsa technique (1976)
    Springer
    Cellular and molecular life sciences 32 (1976), S. 1257-1259 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 μg/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01953080
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Cytogenetics of South American akodont rodents (Cricetidae). V. Segregation of chromosome No. 1 polymorphism inAkodon molinae (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1438-1439 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Akodon molinae is polymorphic with 2n=42, 43, 44, where the metacentric autosome No. 1 is homologous to 2 acrocentrics 1a and 1b. Matings between 2n=43 heterozygotes 1/1a, 1b gave a surplus of 1/1 offspring, a moderate reduction of heterozygous and a strong reduction of homozygous 1a, 1b/1a, 1b offspring. The latter type also has a highly reduced fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01962770
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    The synaptic behaviour of the X and Y chromosomes in the marsupial Monodelphis dimidiata (1975)
    Springer
    Chromosoma 52 (1975), S. 11-25 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The pairing behaviour of the X and Y chromosomes of Monodelphis dimidiata was studied with light and electron microscopy. Pairing of the sex chromosomes is delayed with respect to autosome synapsis. Both the X and the minute Y chromosome show an axis attached by its two ends to the nuclear envelope. Synapsis of the sex chromosomes occurs by the joining of the chromatin sheaths that surround the axes and by a small, three-layered structure close to the nuclear envelope. The X and Y chromosomes remain joined to each other during the diffuse stage and diplotene-diakinesis but they do not show a synaptonemal complex. During the diffuse stage a dense plate is formed at the boundary between the X-Y body and the nuclear envelope. During early metaphase a folded sheet is attached to the periphery of the X-Y body. This sheet is formed by a piece of the nuclear envelope carrying the dense plate and it shows transverse fibrils and a central element similar to synaptonemal-complex remains. No evidence of a non-chiasmate segregation mechanism was observed. Polarization of the axial ends of the sex chromosomes is observed after X-Y synapsis. These important departures from the X-Y pairing pattern of eutherian mammals are discussed and assumed to present a special mechanism for holding the minute Y joined to the X chromosome in this marsupial.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285785
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  • 9
    Electronic Resource
    Electronic Resource
    DNA of Akodon (Rodentia, Cricetidae). I. Biophysical characterization (1979)
    Springer
    Biochemical genetics 17 (1979), S. 297-303 
    Details
    ISSN: 1573-4927
    Keywords: Akodon ; Cricetidae ; DNA characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699–1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The T m values determined in 1 × SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2–43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498970
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    DNA composition in South American camelids I. Characterization and in situ hybridization of satellite DNA fractions (1987)
    Springer
    Genetica 72 (1987), S. 137-146 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The DNA composition and the in situ hybridization of satellite fractions were analysed in the New World camelids llama, alpaca, guanaco and vicuña. In the four camelid forms, it was possible to identify a similar main band DNA and five satellite fractions (I–V) with G+C base contents ranging from 32% to 66%. Satellites II–V from llama were in situ reannealed on chromosomes from the four camelid forms. The results obtained were: (a) the four satellites hybridized with regions of C-banding (centromeric regions of all chromosomes and short arms of some autosomes); (b) in general, homologous hybridizations (llama DNA versus llama chromosomes) were more efficient than heterologous reassociations; there were however three exceptions to this rule (vicuña and alpaca satellite fraction II, chromosome group B; vicuña fraction V, chromosome groups A and B); (c) X chromosomes from the four camelids had satellites III–V but lacked satellite II, (d) no satellite fraction was detected on chromosome Y. The analysis of the in situ hybridization patterns allowed to conclude that most or all C-banded chromosome regions comprise several satellite DNA fractions. It is, moreover, proposed that there is an ample interspecies variation in the number of chromosomes that cross-react with a given satellite fraction. Our data give further support to the close genomic kinship of New World camelids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00123172
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